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Citations & References (13)
Invitrogen™
SNARF™-5F 5-(and-6)-Carboxylic Acid, Acetoxymethyl Ester, Acetate - Special Packaging
細胞透過性レシオメトリックpHインジケーターSNARF™-5F 5-（および-6）-カルボン酸、アセトオキシメチルエステル、酢酸塩（約7.2のpKa）は、酸性および塩基性条件下で、それぞれ黄橙色から深紅色へとpH依存的に有意な発光シフトを示します詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| S23923 | 20 x 50 μg |
製品番号（カタログ番号） S23923
価格（JPY）お問い合わせください ›
117,600
Each
数量:
20 x 50 μg
細胞透過性レシオメトリックpHインジケーターSNARF™-5F 5-（および-6）-カルボン酸、アセトオキシメチルエステル、酢酸塩（約7.2のpKa）は、酸性および塩基性条件下で、それぞれ黄橙色から深紅色へとpH依存的に有意な発光シフトを示します。このpH依存性により、2つの発光波長（通常580 nmおよび640 nm）における色素からの蛍光強度比をpHの定量測定に使用できます。
カルシウム、カリウム、pH、膜電位インジケータなどのイオンインジケータの詳細をご覧ください›
カルシウム、カリウム、pH、膜電位インジケータなどのイオンインジケータの詳細をご覧ください›
研究用にのみ使用できます。診断用には使用いただけません。
仕様
検出法蛍光
染色剤タイプ蛍光色素ベース
インジケーターpHインジケータ
数量20 x 50 μg
出荷条件室温
使用対象 (装置)蛍光顕微鏡, フローサイトメーター, マイクロプレートリーダー
製品ラインSNARF
製品タイプpHインジケータ
Unit SizeEach
組成および保存条件
フリーザー（-5℃～-30℃）に保存。
よくあるご質問（FAQ）
Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?
引用および参考文献 (13)
引用および参考文献
Abstract
Relationships between calcium and pH in the regulation of the slow afterhyperpolarization in cultured rat hippocampal neurons.
Journal:J Neurophysiol
PubMed ID:16885515
'The Ca(2+)-dependent slow afterhyperpolarization (AHP) is an important determinant of neuronal excitability. Although it is established that modest changes in extracellular pH (pH(o)) modulate the slow AHP, the relative contributions of changes in the priming Ca(2+) signal and intracellular pH (pH(i)) to this effect remain poorly defined. To gain a
Potential contribution of a voltage-activated proton conductance to acid extrusion from rat hippocampal neurons.
Journal:Neuroscience
PubMed ID:18201832
'We examined the potential contribution of a voltage-gated proton conductance (gH+) to acid extrusion from cultured postnatal rat hippocampal neurons. In neurons loaded with Ca2+- and/or pH-sensitive fluorophores, transient exposures to 25-139.5 mM external K+ (K+o) or 20 microM veratridine in the presence of 2 mM Ca2+o (extracellular pH (pHo)
Fluorescent indicators for intracellular pH.
Journal:Chem Rev
PubMed ID:19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980.
Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.
Journal:Bioorg Med Chem Lett
PubMed ID:11677123
Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission
Time-domain fluorescence lifetime imaging for intracellular pH sensing in living tissues.
Journal:Anal Bioanal Chem
PubMed ID:18481048
pH sensing in living cells represents one of the most prominent topics in biochemistry and physiology. In this study we performed one-photon and two-photon time-domain fluorescence lifetime imaging with a laser-scanning microscope using the time-correlated single-photon counting technique for imaging intracellular pH levels. The suitability of different commercial fluorescence dyes