SlowFade™ Gold Antifade Mountant

Citations & References (7)

Invitrogen™

SlowFade™ Gold Antifade Mountant

Name: SlowFade™ Gold Antifade Mountant
SKU: S36936

SlowFade Gold Antifade Mountantは、顕微鏡スライド上の顕微鏡スライド上の蛍光標識細胞や組織サンプルに直接塗布する液体封入剤です。蛍光顕微鏡実験中に蛍光色素を退色（光退色）しないように設計された化学成分が含まれています。SlowFade Gold詳細を見る

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製品番号（カタログ番号）	数量
S36936	10 mL
S36937	5 x 2 mL
S36940	2 mL

3 オプション

製品番号（カタログ番号） S36936

価格（JPY）

31,600

Each

お問い合わせください ›

数量:

10 mL

SlowFade Gold Antifade Mountantは、顕微鏡スライド上の顕微鏡スライド上の蛍光標識細胞や組織サンプルに直接塗布する液体封入剤です。蛍光顕微鏡実験中に蛍光色素を退色（光退色）しないように設計された化学成分が含まれています。SlowFade Gold Antifade Mountantはグリセロールベースで、混合や硬化を必要としません。これにより、サンプルの即時観察に最適です。—すぐに使用できるようになっており、サンプルにドロップを適用し、カバースリップを追加して画像を取得するだけです。DAPI核染色の有無にかかわらずご利用いただけます。

主な特性：
•
•すぐに使用可能な液体のイメージング中に色素が色あせないように保護します。マウント
•されたサンプルの即時サンプル表示に最適です。マウントされたサンプルは数週間安定
•しています。—信号強度を維持しますが、クエンチしません
•。Alexa Fluor色素に最適です。

蛍光イメージングのニーズに適した褪色防止用封入剤または光学的透明化試薬をお選びください›

。SlowFade Gold Antifade Mountantは、GFPなど蛍光タンパク質を含むサンプルのマウントには推奨しません。培養細胞や薄い組織切片の蛍光タンパク質や蛍光色素を優れたアンチフェードで保護するには、SlowFade Diamond Antifade Mountantをお勧めします。高分解能、または焦点深度0–500 µmの厚い組織や3D細胞培養のイメージングには、SlowFade Glass Antifade Mountantをお試しください。

お客様の実験ニーズに最適なSlowFade褪色防止剤をお選びください›

研究用途にのみご使用ください。診断目的には使用できません。

仕様

グリーン機能危険性の少ない、持続可能な包装

製品ラインSlowFade

数量10 mL

出荷条件室温

使用対象 (装置)Microscope

形状Liquid

製品タイプMounting Media

溶液タイプAnti-fade Mountant

Unit SizeEach

組成および保存条件

常温での保存をお勧めしますが、冷凍保存も可能です（-5～-30℃）。遮光。

よくあるご質問（FAQ）

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I do not have epoxy or VALAP to seal the coverslip. Do you offer an alternative coverslip sealant?

We offer ProLong Coverslip Sealant (Cat. No. P56128) that can be used to seal the edges of the coverslip and is compatible with both curing and non-curing mountant. The sealant is easy to apply and is brushed on after the mountant has cured, for long preservation of slides. The product page can be found here.

When using a hard-curing mountant, such as ProLong Antifade Mountant, make sure the mountant is fully cured before applying ProLong Coverslip Sealant. Since this sealant can seal moisture under the coverslip, it can interfere with the curing process.

When using a non-curing mountant, such as SlowFade Antifade Mountants, sealing can take place immediately after mounting.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does Slowfade Antifade Mountant require using a nail polish for sealing?

No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (7)

引用および参考文献

Abstract

Reactive oxygen species production via NADPH oxidase mediates TGF-beta-induced cytoskeletal alterations in endothelial cells.

Authors:Hu T, Ramachandrarao SP, Siva S, Valancius C, Zhu Y, Mahadev K, Toh I, Goldstein BJ, Woolkalis M, Sharma K

Journal:Am J Physiol Renal Physiol

PubMed ID:16159901

'Cytoskeletal alterations in endothelial cells have been linked to nitric oxide generation and cell-cell interactions. Transforming growth factor (TGF)-beta has been described to affect cytoskeletal rearrangement in numerous cell types; however, the underlying pathway is unclear. In the present study, we found that human umbilical vein endothelial cells (HUVEC) have ... More

Identification and characterization of small molecules that inhibit intracellular toxin transport.

Authors:Saenz JB, Doggett TA, Haslam DB

Journal:Infect Immun

PubMed ID:17576758

'Shiga toxin (Stx), cholera toxin (Ctx), and the plant toxin ricin are among several toxins that reach their intracellular destinations via a complex route. Following endocytosis, these toxins travel in a retrograde direction through the endosomal system to the trans-Golgi network, Golgi apparatus, and endoplasmic reticulum (ER). There the toxins ... More

Rac-mediated macropinocytosis is a critical route for naked plasmid DNA transfer in mice.

Authors:Fumoto S, Nishi J, Ishii H, Wang X, Miyamoto H, Yoshikawa N, Nakashima M, Nakamura J, Nishida K,

Journal:Mol Pharm

PubMed ID:19492848

We have recently discovered the potential for in vivo naked plasmid DNA (pDNA) transfer into gastric serosal surface cells in mice. As pDNA are huge molecules, the mechanism of gene transfer without carriers and physical forces is of great biological interest. The endocytic route for naked pDNA transfer into gastric ... More

Programmable in situ amplification for multiplexed imaging of mRNA expression.

Authors:Choi HM, Chang JY, Trinh le A, Padilla JE, Fraser SE, Pierce NA,

Journal:Nat Biotechnol

PubMed ID:21037591

In situ hybridization methods enable the mapping of mRNA expression within intact biological samples. With current approaches, it is challenging to simultaneously map multiple target mRNAs within whole-mount vertebrate embryos, representing a significant limitation in attempting to study interacting regulatory elements in systems most relevant to human development and disease. ... More

A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining.

Authors:Wu JS, Luo L

Journal:Nat Protoc

PubMed ID:17487202

This protocol describes a basic method for dissection and immunofluorescence staining of the Drosophila brain at various developmental stages. The Drosophila brain has become increasingly useful for studies of neuronal wiring and morphogenesis in combination with techniques such as the 'mosaic analysis with a repressible cell marker' (MARCM) system, where ... More

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