SlowFade™ Diamond Antifade Mountant with DAPI

Citations & References (11)

Invitrogen™

SlowFade™ Diamond Antifade Mountant with DAPI

Name: SlowFade™ Diamond Antifade Mountant with DAPI
SKU: S36964

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製品番号（カタログ番号）	容量（メートル法）
S36964	5 x 2 mL
S36968	2 mL
S36973	10 mL

3 オプション

製品番号（カタログ番号） S36964

価格（JPY）

39,400

Each

お問い合わせください ›

容量（メートル法）:

5 x 2 mL

SlowFade Diamond退色防止用封入剤は、グリセロールベースの液体封入剤であり、顕微鏡スライド上の蛍光標識細胞や組織サンプルに直接塗布します。可視および赤外スペクトル全域にわたって優れた褪色防止効果を発揮します。SlowFade Diamond封入剤は、ほぼすべての蛍光色素や蛍光タンパク質（GFP、RFP、mCherry など）と組み合わせることで、非常に輝度の高いシグナルと低いバックグラウンドを実現します。

SlowFade Diamond退色防止用封入剤には、以下の特長があります：
•可視および IR スペクトル全体にわたって、蛍光色素および蛍光タンパク質を退色から保護します
• すぐに使用可能な液体で硬化しないため、すぐにサンプルを観察することができます
• Alexa Fluor、FITCやCy3などの従来の色素、GFP、RFP、およびmCherryなどの蛍光タンパク質に最適です
• 蛍光シグナルの維持—ほとんど、またはまったく消光しません

蛍光イメージングのニーズに適した褪色防止用封入剤または光学洗浄試薬を選択 ›

高解像度、または厚みのある組織や3D細胞培養を焦点深度 0～500 µmでイメージングするには、 SlowFade Glass退色防止用封入剤をお試しください。屈折率1.52のRIMS封入剤です。

お客様の実験ニーズに最適な SlowFade退色防止用試薬をお選びください ›

SlowFade Diamond退色防止用封入剤は、すでにミックスされており、すぐに使用可能なドロッパーボトルで、DAPI含有タイプまたは不含タイプがあります。サンプルに一滴垂らしてカバースリップを載せ、イメージングするだけです。カバースリップの端を適切なカバースリップシール剤でシールすると、サンプルを数日から数週間保存できます。ガラスや液浸油に近い屈折率で長期保存する場合は、 ProLong Glass褪色防止用封入剤をお勧めします。SlowFade Diamond封入剤の硬化を必要としない特性により、ラベリング後すぐにサンプルの評価およびイメージングができます。

可視およびIRスペクトルの全域にわたって光退色から保護します
どの蛍光色素や蛍光タンパク質を使用していても、SlowFade Diamond封入剤は光退色に対する最良の保護を提供します。最高クラスの性能は、Alexa Fluor色素、FITC、TRTC、Texasレッド、Cy3、Cy5 などの幅広い蛍光色素との組み合わせで実証されています。SlowFade Diamond封入剤は消光しません。また、バックグラウンドなしでより明るいシグナルを提供できるため、出版に最適な品質の高解像度画像を得ることができます。

イメージング中および迅速な確認のための安定したシグナル
SlowFade Diamond封入剤は、ラベリング直後のサンプル分析に最適なオプションです。蛍光色素や蛍光タンパク質に対する退色防止特性により、長時間のイメージングセッション中（共焦点レーザースキャン照明下でスキャンを繰り返す場合など）や超高解像度顕微鏡を使用するときに、シグナル強度を維持することができます。さらに、染色したサンプルやマウントから余分な水分を除去するだけという使いやすさも兼ね備えています。スライドシール剤を使用すると、スライドを数日間または数週間保存できます。より長いアーカイブが必要な場合は、SlowFade Diamond封入剤をPBS で洗浄し、ProLong Diamond退色防止用封入剤でサンプルを再マウントできます。

鮮明な画像を得るための優れた屈折率を実現
最新の高解像度イメージングアプリケーションにおいて、屈折率（RI）マッチングが不可欠です。SlowFade™ Diamond封入剤の屈折率は1.42で、培養細胞や組織の薄いスライスにお勧めです（厚さ10 μm未満）。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

数量5 x 2 mL

製品ラインSlowFade

製品タイプAntifade Mountant

試薬タイプマウント溶液、アンチフェード溶液

容量（メートル法）5 x 2 mL

Unit SizeEach

組成および保存条件

各2 mL試薬を含むドロッパーボトル5本が含まれています。

•2～8℃で保管するか、-5～–30℃で冷凍保管します。
•遮光。
• 受け取り後6カ月間は安定しています。

よくあるご質問（FAQ）

What is the difference between ProLong and SlowFade antifade reagents?

Our ProLong antifade reagents dispense as a liquid that will solidify upon the evaporation of water. SlowFade antifade reagents remain liquid. If you are going to image right away and then dispose of your sample, you do not need a mountant that hardens, such as the SlowFade reagents. If you wish to archive your slide for more than a day, you will want a mounting medium that hardens (or “cures”). This hardening will limit the off-rates of various dye-conjugated antibodies and provides a better refractive index. Also, there will be a lower diffusion rate of free radicals, limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Some antifade mounting media stay as liquid whereas others harden. What is the benefit of having one that hardens?

If you are going to image right away and then dispose of your sample, you probably want a mountant that does not harden. If you wish to archive your slide for more than a day, you want a mountant that hardens (or "cures"). This hardening will slow or prevent off-rate of your dye or conjugate and often produces a better refractive index. Secondary sealing is usually not necessary. Also there will be lower diffusion of free radicals, thus limiting photobleaching.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I do not have epoxy or VALAP to seal the coverslip. Do you offer an alternative coverslip sealant?

We offer ProLong Coverslip Sealant (Cat. No. P56128) that can be used to seal the edges of the coverslip and is compatible with both curing and non-curing mountant. The sealant is easy to apply and is brushed on after the mountant has cured, for long preservation of slides. The product page can be found here.

When using a hard-curing mountant, such as ProLong Antifade Mountant, make sure the mountant is fully cured before applying ProLong Coverslip Sealant. Since this sealant can seal moisture under the coverslip, it can interfere with the curing process.

When using a non-curing mountant, such as SlowFade Antifade Mountants, sealing can take place immediately after mounting.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does Slowfade Antifade Mountant require using a nail polish for sealing?

No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to mount my dye-labeled cells in an antifade mounting medium to keep the dyes from photobleaching. Which mounting medium do you recommend?

As dyes are illuminated for imaging, they will fade, or “photobleach”, leading to unwanted dimming and lower detection efficiency over time. An antifade mounting medium can greatly reduce photobleaching. If you wish to label live cells, use of ProLong Live Antifade Reagent is helpful. If you wish to mount fixed cells after labeling, and then image immediately and then discard, SlowFade Diamond Antifade Mountant stays liquid but has good refractive index. If you wish to mount your sample and then archive the slides, ProLong Diamond Antifade Mountant will harden to a better refractive index and allow for archiving of the sample for up to several weeks, or even months. Unlike other antifade mounting media, these work well with fluorescent proteins for immediate viewing (archiving fluorescent proteins is not possible), and they are packaged with or without DAPI. More information on these can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/fluorescence-microscopy-and-immunofluorescence-if/mounting-medium-antifades/prolong-gold-antifade.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all SlowFade™ Diamond Antifade Mountant with DAPI, 5 x 2 mL FAQs

引用および参考文献 (11)

引用および参考文献

Abstract

Human Primordial Germ Cells Are Specified from Lineage-Primed Progenitors.

Authors:Chen D, Sun N, Hou L, Kim R, Faith J, Aslanyan M, Tao Y, Zheng Y, Fu J, Liu W, Kellis M, Clark A

Journal:Cell Rep

PubMed ID:31875561

'In vitro gametogenesis is the process of making germline cells from human pluripotent stem cells. The foundation of this model is the quality of the first progenitors called primordial germ cells (PGCs), which in vivo are specified during the peri-implantation window of human development. Here, we show that human PGC (hPGC) specification ... More

SosA inhibits cell division in Staphylococcus aureus in response to DNA damage.

Authors:Bojer MS, Wacnik K, Kjelgaard P, Gallay C, Bottomley AL, Cohn MT, Lindahl G, Frees D, Veening JW, Foster SJ, Ingmer H

Journal:Mol Microbiol

PubMed ID:31290194

'Inhibition of cell division is critical for viability under DNA-damaging conditions. DNA damage induces the SOS response that in bacteria inhibits cell division while repairs are being made. In coccoids, such as the human pathogen, Staphylococcus aureus, this process remains poorly studied. Here, we identify SosA as the staphylococcal SOS-induced ... More

Light needle microscopy with spatially transposed detection for axially resolved volumetric imaging.

Authors:Kozawa Y, Sato S

Journal:Sci Rep

PubMed ID:31406209

'The demand for rapid three-dimensional volumetric imaging is increasing in various fields, including life science. Laser scanning fluorescence microscopy has been widely employed for this purpose; however, a volumetric image is constructed by two-dimensional image stacking with a varying observation plane, ultimately limiting the acquisition speed. Here we propose a ... More

Nanoscale Surveillance of the Brain by Microglia via cAMP-Regulated Filopodia.

Authors:Bernier LP, Bohlen CJ, York EM, Choi HB, Kamyabi A, Dissing-Olesen L, Hefendehl JK, Collins HY, Stevens B, Barres BA, MacVicar BA

Journal:Cell Rep

PubMed ID:31167136

'Microglia, the brain''s immune cells, maintain homeostasis and sense pathological changes by continuously surveying the parenchyma with highly motile large processes. Here, we demonstrate that microglia also use thin actin-dependent filopodia that allow fast nanoscale sensing within discrete regions. Filopodia are distinct from large processes by their size, speed, and ... More

Spatial organization of the somatosensory cortex revealed by osmFISH.

Authors:Codeluppi S, Borm LE, Zeisel A, La Manno G, van Lunteren JA, Svensson CI, Linnarsson S

Journal:Nat Methods

PubMed ID:30377364

Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type-specific markers simultaneously on large tissue areas. Here, we describe ... More

View all SlowFade™ Diamond Antifade Mountant with DAPI, 5 x 2 mL citations