SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO
SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO
Citations & References (262)
Invitrogen™

SYTOX™ Green Nucleic Acid Stain - 5 mM Solution in DMSO

SYTOX™グリーン核酸染色剤は、生細胞には浸透しない優れた緑色蛍光核および染色体対比染色剤であり、集団内の死細胞の有用なインジケーターとなります。室温で安定した、すぐに使用できる溶液としてもご利用いただけます。NucGreen Dead 488 Readyプローブ試薬。他のReady詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
S7020250 μL
製品番号（カタログ番号） S7020
価格（JPY）
74,100
Each
お問い合わせください ›
数量:
250 μL
SYTOX™グリーン核酸染色剤は、生細胞には浸透しない優れた緑色蛍光核および染色体対比染色剤であり、集団内の死細胞の有用なインジケーターとなります。

室温で安定した、すぐに使用できる溶液としてもご利用いただけます。NucGreen Dead 488 Readyプローブ試薬。
他のReady Probes Ready-to-useイメージング試薬およびアクセサリをご覧ください。›

•  生細胞に浸透しません。
•  励起／発光：504/523 nm
•  488アルゴンイオンレーザーで使用
•  哺乳類細胞とグラム陽性菌とグラム陰性菌の両方で機能します

シンプルな生存率の決定
SYTOX™Greenを使用すると、フローサイトメーター、蛍光顕微鏡、蛍光光度計、または蛍光マイクロプレートリーダーを使用して細胞の生存率をすばやく測定できます。無傷な膜を通過することはありませんが、死細胞の特徴である、損傷した膜を容易に通過します。核酸の結合時に>500倍の蛍光増強が見られるため、SYTOX™ Greenで死細胞を短時間インキュベートすると、488 nmのアルゴンイオンレーザーまたはその他の450–490 nm光源で励起した場合に、発光ピークが523 nmの明るい緑色蛍光が生じます。死細胞で生成されるシグナルの優れた輝度により、SYTOX™グリーンは哺乳類細胞だけでなく、グラム陽性細菌とグラム陰性細菌の両方の生存率を測定するのに特に有用です。

細胞生存率の染色およびアッセイのInvitrogenファミリーの一部
死細胞用のSYTOX™染色剤は、赤、青、オレンジなどのさまざまな色が用意されています。さらに、Invitrogenは、アポトーシス、細胞生存率、および代謝のためにSYTOX™染色を多数のアッセイに組み込みました。

研究用途にのみご使用ください。動物またはヒトの治療または診断用には使用できません。

関連リンク
•  すべてのSYTOX™製品の詳細をご覧ください。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
色Green
検出法蛍光
染色剤タイプ細胞透過性
発光523 nm
励起波長域504⁄523
使用対象 (装置)フローサイトメーター
形状溶液
フォーマットチューブ
製品ラインSYTOX
数量250 μL
出荷条件室温
容量（メートル法）250 μL
標識タイプFluorescent Dye
製品タイプ核酸染色
SubCellular Localization核、核酸, Nucleus
Unit SizeEach
組成および保存条件
SYTOXグリーン核酸染色液(DMSO中の5 mM溶液)のバイアルが1つ含まれています。

フリーザー(-5∼-30度)に保存し、遮光してください。

よくあるご質問（FAQ）

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to mount my Qdot secondary-labeled tissue samples in HistoMount mounting medium, but my Qdot 705 conjugate overlaps with your Qnuclear Deep Red nuclear label. What other nuclear label would you recommend, that is compatible with HistoMount mounting medium?

We recommend using SYTOX Green stain, which you can image using a FITC filter and we have shown to be compatible with HistoMount mounting medium. Some have used DAPI, but there have been some issues with slight quenching and spectral shifting of DAPI into green or even red wavelengths.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (262)

引用および参考文献
Abstract
Use of SYTOX green dye in the flow cytometric analysis of bacterial phagocytosis.
Authors:Gaforio JJ, Serrano MJ, Ortega E, Algarra I, Alvarez de Cienfuegos G
Journal:Cytometry
PubMed ID:12116370
BACKGROUND: Fluorescein isothiocyanate (FITC) is used widely to label the targets used in flow cytometric phagocytosis assays. Unfortunately, the fluorescence intensity of phagocytosed FITC-labeled targets is influenced by changes in intracellular pH level, making quantitative measurements with this fluorophore problematic. We describe the use of SYTOX green nucleic acid stain ... More
Identification and characterization of two subpopulations of Encephalitozoon intestinalis.
Authors:Hoffman RM, Marshall MM, Polchert DM, Jost BH
Journal:Appl Environ Microbiol
PubMed ID:12902292
Microsporidia are obligate intracellular protozoa that have been shown to be pathogenic to most living creatures. The development of in vitro cell culture propagation methods has provided researchers with large numbers of spores and facilitated the study of these organisms. Here, we describe heterogeneity within cell culture-propagated Encephalitozoon intestinalis suspensions. ... More
Unique catabolic pathway of glycosphingolipids in a hydrozoan, Hydra magnipapillata, involving endoglycoceramidase.
Authors:Horibata Y, Sakaguchi K, Okino N, Iida H, Inagaki M, Fujisawa T, Hama Y, Ito M
Journal:J Biol Chem
PubMed ID:15320336
Endoglycoceramidase (EGCase; EC 3.2.1.123) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. We detected strong EGCase activity in animals belonging to Cnidaria, Mollusca, and Annelida and cloned the enzyme from a hydra, Hydra magnipapillata. The hydra EGCase, consisting of 517 amino acid ... More
Cell death during ischemia: relationship to mitochondrial depolarization and ROS generation.
Authors:Levraut J, Iwase H, Shao ZH, Vanden Hoek TL, Schumacker PT
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:12388276
Ischemia-reperfusion injury induces cell death, but the responsible mechanisms are not understood. This study examined mitochondrial depolarization and cell death during ischemia and reperfusion. Contracting cardiomyocytes were subjected to 60-min ischemia followed by 3-h reperfusion. Mitochondrial membrane potential (DeltaPsi(m)) was assessed with tetramethylrhodamine methyl ester. During ischemia, DeltaPsi(m) decreased to ... More
Up-regulation of cyclooxygenase-2 and apoptosis resistance by p38 MAPK in hypericin-mediated photodynamic therapy of human cancer cells.
Authors:Hendrickx N, Volanti C, Moens U, Seternes OM, de Witte P, Vandenheede JR, Piette J, Agostinis P
Journal:J Biol Chem
PubMed ID:14557269
Photodynamic Therapy (PDT) is an approved anticancer therapy that kills cancer cells by the photochemical generation of reactive oxygen species following absorption of visible light by a photosensitizer, which selectively accumulates in tumors. We report that hypericin-mediated PDT of human cancer cells leads to up-regulation of the inducible cyclooxygenase-2 (COX-2) ... More
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