XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)

XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2<i>H</i>-Tetrazolium-5-Carboxanilide)

Citations & References (62)

Invitrogen™

XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)

XTTは、酸化還元電位の関数として細胞生存率を評価するために使用されます。呼吸活性を示す細胞は、水溶性XTTを、水溶性のオレンジ色のホルマザン生成物に変換します。MTTとは異なり、XTTは定量前に可溶化する必要がないため、多くの生存率アッセイプロトコルでアッセイ時間を短縮できます。さらに、XTT還元アッセイの感度は詳細を見る

製品番号（カタログ番号）	数量
X6493	100 mg

製品番号（カタログ番号） X6493

価格（JPY）

23,100

Exklusiv online

Ends: 27-Mar-2026

38,600

割引額 15,500 (40%)

100 mg

お問い合わせください ›

100 mg

XTTは、酸化還元電位の関数として細胞生存率を評価するために使用されます。呼吸活性を示す細胞は、水溶性XTTを、水溶性のオレンジ色のホルマザン生成物に変換します。MTTとは異なり、XTTは定量前に可溶化する必要がないため、多くの生存率アッセイプロトコルでアッセイ時間を短縮できます。さらに、XTT還元アッセイの感度は、MTT還元アッセイと同等以上であることが報告されています。

研究用にのみ使用できます。診断用には使用いただけません。

細胞タイプ真核細胞

検出法比色法

染色剤タイプその他の標識または色素

フォーマット96-ウェルプレート

数量100 mg

出荷条件室温

使用対象 (装置)マイクロプレートリーダー

製品タイプXTT

Unit Size100 mg

組成および保存条件

フリーザー（-5℃～-30℃）に保存。

よくあるご質問（FAQ）

MTT cell proliferation assays require cellular metabolism to turn over the reagent, and thus only live cells will be counted. Is this true for CyQUANT Direct as well?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

If I used XTT (Cat. No. X6493) with an electron coupling reagent, would I get the same results as if I used the CyQUANT XTT Cell Viability Assay kit (Cat. No. X12223)?

Both products use the same reagent, XTT. In theory, the results of any optimized assay using XTT (Cat. No. X6493) may be comparable to using the CyQUANT XTT Cell Viability Assay kit (Cat. No. X12223). Variations may arise due to the type of electron coupling reagent used, the final concentration of XTT, and any assay-specific requirements of the solutions used in resolubilizing the reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When should I use the XTT reagent alone (Cat No. X6493) versus the CyQUANT XTT Cell Viability Assay (Cat No. X12223)?

For the CyQUANT XTT Cell Viability Assay kit (Cat No. X12223), the amount of reagents, the formulation of the solutions, and the identity of the Electron Coupling Reagent are proprietary information. On the other hand, XTT (Cat. No. X6493) may be resolubilized in either water, buffer, media, or DMSO to a desired stock concentration. We would recommend using XTT, Cat No. X6493 if you need to optimize the final working concentration, if the type of buffer/solvent used to resolubilize the reagent is critical to the study, if you elect not to use an electron coupling reagent, or if you'd like to use a known electron coupling reagent of a defined concentration.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between XTT (Cat No. X6493) and CyQUANT XTT Cell Viability Assay (Cat No. X12223)?

XTT (Cat No. X6493) is 100 mg of XTT in solid form; you must resolubilize this into an aqueous solution or DMSO prior to use.

CyQUANT XTT Cell Viability Assay (Cat No. X12223) is a kit that provides two components: 10 vials each of the XTT Reagent and Electron Coupling Reagent. Both kit components are in solution in a ready-to-use format; mix 1 vial of the XTT Reagent and 1 vial of the Electron Coupling Reagent immediately before use.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (62)

引用および参考文献

Abstract

Endoglin is a component of the transforming growth factor (TGF)-beta receptor complex of human pre-B leukemic cells.

Authors:Zhang H, Shaw AR, Mak A, Letarte M

Journal:J Immunol

PubMed ID:8543807

'Endoglin was first identified on a cell line derived from pre-B acute lymphoblastic leukemia. This 180-kDa homodimeric glycoprotein was then shown to be primarily expressed on endothelial cells and to bind the beta 1 and beta 3 isoforms of TGF-beta with high affinity. We now demonstrate that pre-B leukemic cells ... More

9-Nitrocamptothecin inhibits HIV-1 replication in human peripheral blood lymphocytes: a potential alternative for HIV-infection/AIDS therapy.

Authors:Hung CL, Doniger J, Palini A, Snyder SW, Radonovich MF, Brady JN, Pantazis P, Sadaie MR

Journal:J Med Virol

PubMed ID:11424110

'The ability of the anti-cancer drug, 9-Nitrocamptothecin (9NC), to inhibit replication of HIV-1 in clinically relevant primary lymphocytic cells was studied. Primary peripheral blood lymphocytes (PBLs) from a non-infected donor were freshly infected with HIV-1 and treated with 9NC by using three different treatment schedules. Cells were monitored for cytotoxicity ... More

In vitro effects of antiviral agents on human keratocytes.

Authors:Moreira LB, Oliveira C, Seitz B, LaBree L, McDonnell PJ

Journal:Cornea

PubMed ID:11189008

'PURPOSE: To study the effects of antiviral agents on human keratocytes in vitro. METHODS: Cultured human keratocytes were incubated with either ganciclovir, idoxuridine, trifluridine, or cidofovir at concentrations from 0.0001 to 10 mg/mL. Phase-contrast microscopy and XTT (sodium [2,3-bis [2-methoxy-4-nitro-5-sulphophenyl]-2h-tetrazolium-5-carboxanilide, inner salt) colorimetric assay were performed after 24, 48, and ... More

The interaction of human monocytes, monocyte-derived macrophages, and polymorphonuclear neutrophils with caspofungin (MK-0991), an echinocandin, for antifungal activity against Aspergillus fumigatus.

Authors:Chiller T, Farrokhshad K, Brummer E, Stevens DA

Journal:Diagn Microbiol Infect Dis

PubMed ID:11248522

'The collaboration between human effector cells and caspofungin (MK-0991), a 1,3-beta-D glucan synthase inhibitor, was studied for antifungal activity against Aspergillus fumigatus. Caspofungin was co-cultured for 24h with either human monocytes (Monos), monocyte-derived macrophages (MDM), or polymorphonuclear neutrophils (PMN) against germlings of A. fumigatus and antifungal activity assessed using the ... More

In vitro growth and analysis of Candida biofilms.

Authors:Chandra J, Mukherjee PK, Ghannoum MA,

Journal:Nat Protoc

PubMed ID:19180075

'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion ... More

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