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Invitrogen

IFN gamma Monoclonal Antibody (NIB42), eBioscience™

12 Published Figures
32 References
View all (204) IFN gamma antibodies

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Product Details

14-7318-85

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

-
View 11 publications 11 publications

ELISA (ELISA)

2-8 µg/mL
View 7 publications 7 publications

Neutralization (Neu)

-
View 8 publications 8 publications

Functional Assay (Functional)

-
View 2 publications 2 publications

Inhibition Assays (Inhibition)

-
View 2 publications 2 publications

Affinity Purification (AP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

NIB42

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Amount

500 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468476

Product Specific Information

Description: The NIB42 antibody reacts with human interferon-gamma (IFN-gamma). The NIB42 antibody is a neutralizing antibody. IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.

Applications Reported: The NIB42 antibody has been reported for use in capture of human IFN gamma by ELISA, and for neutralizing IFN gamma bioactivity. Use Functional Grade purified for functional assays Product # 16-7318).

Applications Tested: The NIB42 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interferon-gamma (IFN-g) in combination with the biotin 4S.B3 ( Product # 13-7319-81) antibody for detection and recombinant human IFNg (Product # 29-8319-65) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.For ELISPOT Capture, the alternative clone MD-1 is recommended.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cytokine; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNg; IFNγ; Immune interferon; Interferon; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g; unnamed protein product

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Gene Aliases: IFG; IFI; IFNG; IMD69

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UniProt ID: (Human) P01579

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Entrez Gene ID: (Human) 3458

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Function(s)
cytokine activity interferon-gamma receptor binding protein binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter microglial cell activation positive regulation of cytokine production adaptive immune response macrophage activation involved in immune response positive regulation of T cell cytokine production apoptotic process immune response humoral immune response cell surface receptor signaling pathway JAK-STAT cascade positive regulation of cell proliferation response to virus positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration macrophage differentiation negative regulation of epithelial cell differentiation positive regulation of protein complex assembly negative regulation of interleukin-17 production positive regulation of chemokine production positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-23 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response positive regulation of peptidyl-serine phosphorylation of STAT protein positive regulation of smooth muscle cell apoptotic process Fc-gamma receptor signaling pathway involved in phagocytosis interleukin-2-mediated signaling pathway type III interferon-mediated signaling pathway positive regulation of protein import into nucleus positive regulation of tyrosine phosphorylation of STAT protein positive regulation of MHC class II biosynthetic process positive regulation of nitric oxide biosynthetic process positive regulation of osteoclast differentiation positive regulation of cell adhesion positive regulation of glycolytic process negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter astrocyte activation negative regulation of smooth muscle cell proliferation positive regulation of inflammatory response positive regulation of phagocytosis positive regulation of neurogenesis regulation of insulin secretion positive regulation of membrane protein ectodomain proteolysis positive regulation of transport defense response to virus interferon-gamma-mediated signaling pathway positive regulation of fructose 1,6-bisphosphate metabolic process positive regulation of vitamin D biosynthetic process positive regulation of protein serine/threonine kinase activity positive regulation of protein deacetylation extrinsic apoptotic signaling pathway receptor signaling pathway via STAT cellular response to virus neuroinflammatory response negative regulation of beta-amyloid clearance positive regulation of glutamate receptor signaling pathway positive regulation of cellular respiration positive regulation of beta-amyloid formation positive regulation of protein localization to plasma membrane positive regulation of exosomal secretion positive regulation of iron ion import across plasma membrane positive regulation of core promoter binding positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
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