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Invitrogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
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FIGURE: 1 / 99
Rabbit IgG (H+L) Secondary Antibody (31460) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1) and Hep G2 (Lane 2). The blots were probed with Anti-CacyBP Rabbit Polyclonal Antibody (Product # 720326, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # 31460) at dilutions 1:10,000 (Fig. 1), 1:100,000 (Fig. 2) and 1:200,000 (Fig. 3). A 30 kDa b... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
31460
Applications
Tested Dilution
Western Blot (WB)
1:10,000-1:200,000
Immunohistochemistry (Paraffin) (IHC (P))
Assay-dependent
ELISA (ELISA)
1:5,000-1:10,000
Immunoprecipitation (IP)
1:500-1:5,000
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Purified Rabbit IgG, whole molecule
Conjugate
HRP
HRP
HRP
Form
Lyophilized
Concentration
0.8 mg/mL once reconstituted
Amount
1.6 mg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA, 50mM sucrose
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Product #31460 has been validated for use in Western blot, immunohistochemistry (IHC), ELISA, and immunoprecipitation (IP) applications.
This product reacts with the heavy chains of rabbit IgG and the light chains common to most rabbit immunoglobulins. It does not react with non-immunoglobulin serum proteins. However, cross-reactivity may occur with immunoglobulins from other species and with SuperBlock™ Blocking Buffers.
Storage Conditions
Store at 2-8°C prior to reconstitution.
Reconstitution and Storage Guidelines
Short-term storage (≤1 week): Reconstitute with 2 mL distilled water. Store at 2-8°C.
Long-term storage: Reconstitute with 1 mL distilled water, add 1 mL glycerol, and store at -20°C. Stable for up to 1 year.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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