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Invitrogen
Claudin 18 Recombinant Rabbit Monoclonal Antibody (34H14L15)
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FIGURE: 1 / 27
Claudin 18 Antibody (700178) in ICC/IF
Immunofluorescent analysis of Claudin-18 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Claudin-18 Recombinant Rabbit Monoclonal Antibody (Product # 700178) at a dilution of 1:500 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Ale... View More
Product Details
700178
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Amphibian,
Dog,
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
34H14L15
Immunogen
A peptide corresponding to amino acids 224-237 of P56856.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with chimpanzee, equine, rat and Rhesus monkey based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.The immunogenic sequence for this antibody is GFKASTGFGSNTKNH, and the antibody is predicted to react with chimpanzee, equine, rat, and Rhesus monkey based on sequence identity and similarity.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: claudin 18-like; Claudin-18; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J; unnamed protein product
Gene Aliases: CLDN18; SFTA5; SFTPJ; UNQ778/PRO1572
UniProt ID: (Human) P56856, (Mouse) P56857, (Rat) Q5I0E5
Entrez Gene ID: (Human) 51208, (Mouse) 56492, (Rat) 315953
cell adhesion
negative regulation of tumor necrosis factor-mediated signaling pathway
calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules
protein localization to nucleus
organ growth
epithelial fluid transport
response to ethanol
negative regulation of bone resorption
lung alveolus development
digestive tract development
epithelial cell proliferation
bicellular tight junction assembly
cellular response to estrogen stimulus
tight junction organization
negative regulation of protein localization to nucleus
negative regulation of osteoclast development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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