Search
Invitrogen
Phospho-FAK (Tyr397) Recombinant Rabbit Monoclonal Antibody (31H5L17)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 32
Phospho-FAK (Tyr397) Antibody (700255) in ICC/IF
Immunofluorescent analysis of Phospho-FAK pTyr397 in A549 (top) and HeLa cells (bottom) using a Phospho-FAK pTyr397 recombinant rabbit monoclonal antibody (Product # 700255) at a dilution of 1:2000 (A549) and 1:1000 (HeLa) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Hoechst only (left), AF488 signal only (middle) and composite image ... View More
Product Details
700255
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Dog,
Fruit fly,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
31H5L17
Immunogen
A peptide corresponding to amino acids 390-401 of Q05397.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with mouse, rat, primate, bovine, equine, canine, chicken, opossum, zebra finch, zebrafish, Xenopus, and pufferfish based on sequence homology.
This antibody was tested in IHC on breast, gastric, and thyroid carcinoma tissue and on HeLa and A549 cells in IF applications. This antibody has been used as a detector in a sandwich ELISA format.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 2.7.10.2; FADK 1; FAK-Del33; exon 33 deletion splicing mutant; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2; unnamed protein product
Gene Aliases: FADK; FADK 1; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2
UniProt ID: (Human) Q05397, (Mouse) P34152
Entrez Gene ID: (Human) 5747, (Mouse) 14083
nucleotide binding
actin binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein tyrosine phosphatase activity
integrin binding
protein binding
ATP binding
JUN kinase binding
kinase activity
transferase activity
protein kinase binding
protein phosphatase binding
SH2 domain binding
molecular function activator activity
signal transducer activity
receptor binding
transferase activity, transferring phosphorus-containing groups
protein complex binding
identical protein binding
phosphatidylinositol 3-kinase binding
MAPK cascade
microtubule cytoskeleton organization
angiogenesis
blood vessel development
vasculogenesis
neuron migration
regulation of protein phosphorylation
positive regulation of protein phosphorylation
protein phosphorylation
movement of cell or subcellular component
cell adhesion
signal complex assembly
epidermal growth factor receptor signaling pathway
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
JNK cascade
multicellular organism development
positive regulation of cell proliferation
regulation of cell shape
regulation of epithelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
positive regulation of smooth muscle cell migration
phosphorylation
peptidyl-tyrosine phosphorylation
central nervous system neuron axonogenesis
negative regulation of cell-cell adhesion
regulation of cell adhesion
extracellular matrix organization
positive regulation of cell growth
positive regulation of cell migration
negative regulation of cell migration
cellular chloride ion homeostasis
regulation of cell adhesion mediated by integrin
peptidyl-tyrosine autophosphorylation
establishment of nucleus localization
regulation of cell proliferation
negative regulation of apoptotic process
endothelial cell migration
innate immune response
regulation of osteoblast differentiation
positive regulation of cell adhesion
positive regulation of protein kinase activity
positive regulation of vasodilation
negative regulation of organ growth
protein autophosphorylation
ephrin receptor signaling pathway
positive regulation of smooth muscle cell proliferation
positive regulation of phagocytosis
negative regulation of axonogenesis
positive regulation of synaptic transmission
regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
negative regulation of synapse assembly
positive regulation of glial cell proliferation
growth hormone receptor signaling pathway
cellular response to transforming growth factor beta stimulus
regulation of substrate adhesion-dependent cell spreading
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of anoikis
placenta development
regulation of cell-matrix adhesion
heart morphogenesis
axon guidance
regulation of endothelial cell migration
positive regulation of epithelial cell migration
positive regulation of epithelial to mesenchymal transition
positive regulation of macrophage chemotaxis
positive regulation of fibroblast migration
cell migration
establishment of cell polarity
cell differentiation
detection of muscle stretch
netrin-activated signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
regulation of GTPase activity
regulation of cell differentiation
vascular endothelial growth factor receptor signaling pathway
cell motility
regulation of multicellular organismal process
regulation of cytoskeleton organization
positive regulation of wound healing
vascular endothelial cell response to oscillatory fluid shear stress
positive regulation of macrophage proliferation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support