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Invitrogen
Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ 488
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FIGURE: 1 / 8
Mouse IgG (H+L) Secondary Antibody (A27023) in ICC/IF
Immunofluorescence analysis of Rabbit anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488 was performed using SH-SY5Y (positive model) and T-47D (negative model) cells stained with Nestin Monoclonal Antibody (10C2), eBioscience™ (Product # 14-9843-80). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, blocked w... View More
Product Details
A27023
Applications
Tested Dilution
Western Blot (WB)
1:2,500-1:5,000
Immunocytochemistry (ICC/IF)
1:1,000-1:2,000
Flow Cytometry (Flow)
1-3 µg/mL
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Secondary Antibody
Immunogen
Recombinant full-length protein
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
1 mg
Purification
Gravity column chromatography
Storage buffer
PBS
Contains
5mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
The sensitivity and specificity of each lot is confirmed using ELISA.
Minimal cross-reactivity with goat, rat, human, bovine, guinea pig, and donkey IgG is observed.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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