Search
Invitrogen
ERK3 Monoclonal Antibody (5E1)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 9
ERK3 Antibody (MA1-101) in ICC/IF
Immunofluorescent analysis of ERK3 (green) showing positive staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an ERK3 monoclonal antibody (Product # MA1-101) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated... View More
Product Details
MA1-101
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
5E1
Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-101 has been successfully used in Western blot, immunohistochemistry and immunfluorescence applications on human and mouse samples.
Target Information
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. Tissue specificity: Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: DKFZp686F03189; ERK-3; Erk-3 related; Extracellular signal-regulated kinase 3; extracellular signal-regulated kinase, p97; MAP kinase 6; MAP kinase isoform p97; MAPK 6; mitogen activated protein kinase 4; mitogen activated protein kinase 6; Mitogen-activated protein kinase 6; p97-MAPK; protein kinase, mitogen activated kinase 4; protein kinase, mitogen-activated 5; protein kinase, mitogen-activated 6; unnamed protein product
Gene Aliases: 2610021I23Rik; D130053K17Rik; ERK3; HsT17250; Mapk4; MAPK6; Mapk63; p97MAPK; Prkm4; PRKM6
UniProt ID: (Human) Q16659, (Mouse) Q61532
Entrez Gene ID: (Human) 5597, (Mouse) 50772
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein kinase binding
protein heterodimerization activity
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support