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Invitrogen
CCR7 Monoclonal Antibody (4B12)
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FIGURE: 1 / 3
CCR7 Antibody (MA1-163) in WB
Western blot analysis of CCR7 was performed by loading the indicated amounts of Jurkat whole cell lysate and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% nonfat dry milk in TBST for at least 1 hour at room temperature. CCR7 was detected at ~43 kD using a CCR7 monoclonal antibody (Product # MA1-163) at a dilution of 1:500 in blocking buffer overnight at 4C o... View More
Product Details
MA1-163
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
4B12
Immunogen
Rat basophilic leukaemia (RBL)-2H3 transfectants expressing mouse CCR7
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-163 has been validated for flow cytometry on primary mouse splenocytes. Optimal staining is observed when incubating the cells with antibody at 37C for at least 30 minutes.
MA1-163 has been validated for Western blot on Jurkat cell lysates.
Target Information
CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; chemokine (C-C) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; G-protein coupled receptor; CCR7; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor; unnamed protein product
Gene Aliases: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; Ebi1h; EVI1
UniProt ID: (Human) P32248, (Mouse) P47774
Entrez Gene ID: (Human) 1236, (Mouse) 12775
G-protein coupled receptor activity
chemokine receptor activity
C-C chemokine receptor activity
chemokine (C-C motif) ligand 19 binding
chemokine (C-C motif) ligand 21 binding
C-C motif chemokine 19 receptor activity
C-C motif chemokine 21 receptor activity
signal transducer activity
protein binding
establishment of T cell polarity
myeloid dendritic cell chemotaxis
lymphocyte chemotaxis across high endothelial venule
positive regulation of dendritic cell antigen processing and presentation
regulation of tolerance induction to self antigen
positive regulation of hypersensitivity
positive regulation of humoral immune response
chemotaxis
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
activation of JUN kinase activity
positive regulation of macrophage chemotaxis
positive regulation of T cell chemotaxis
actin cytoskeleton organization
response to lipopolysaccharide
regulation of interferon-gamma production
positive regulation of interleukin-12 production
positive regulation of tumor necrosis factor production
response to prostaglandin E
chemokine (C-C motif) ligand 19 signaling pathway
chemokine (C-C motif) ligand 21 signaling pathway
positive regulation of T cell proliferation
positive regulation of GTPase activity
negative thymic T cell selection
positive regulation of T-helper 1 cell differentiation
positive regulation of receptor-mediated endocytosis
lymph node development
regulation of interleukin-1 beta secretion
positive regulation of interleukin-1 beta secretion
positive regulation of phagocytosis
positive regulation of T cell receptor signaling pathway
positive regulation of filopodium assembly
chemokine-mediated signaling pathway
cellular response to cytokine stimulus
response to nitric oxide
interleukin-12 secretion
positive regulation of neutrophil chemotaxis
lymphocyte migration into lymph node
mature conventional dendritic cell differentiation
negative regulation of leukocyte apoptotic process
positive regulation of cell motility
positive regulation of thymocyte migration
positive regulation of dendritic cell chemotaxis
positive regulation of immunological synapse formation
positive regulation of T cell costimulation
positive regulation of glycoprotein biosynthetic process involved in immunological synapse formation
regulation of dendritic cell dendrite assembly
positive regulation of cell-matrix adhesion
dendritic cell chemotaxis
positive regulation of cytosolic calcium ion concentration
calcium-mediated signaling
positive regulation of actin filament polymerization
positive regulation of pseudopodium assembly
ruffle organization
regulation of Cdc42 protein signal transduction
regulation of interleukin-1 beta production
negative regulation of interleukin-12 production
positive regulation of Rac protein signal transduction
CCL19-activated CCR7 signaling pathway
CCL21-activated CCR7 signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of cell adhesion
positive regulation of JNK cascade
homeostasis of number of cells
release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
cell chemotaxis
positive regulation of ERK1 and ERK2 cascade
cellular response to prostaglandin E stimulus
positive regulation of phospholipase C/protein kinase C signal transduction
negative regulation of dendritic cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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