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Invitrogen
SRC1 Monoclonal Antibody (1135/H4)
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FIGURE: 1 / 9
SRC1 Antibody (MA1-840) in WB
Western blot analysis of SRC1 was performed by loading 25 µg of LNCaP (lane 1), PC-3 (lane 2) and NIH-3T3 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a SRC1 monoclonal antibody (Product # MA1-840) at a dilution of 1:2000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting S... View More
Product Details
MA1-840
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1135/H4
Immunogen
Synthetic peptide corresponding to residues 477-947 of human SRC-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-840 detects steroid receptor coactivator-1 (SRC-1) from mouse and primate tissues and cells as well as recombinant human SRC-1 protein.
MA1-840 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot MA1-840 detects an ~165 kDa protein representing SRC-1 in COS cell extract. Also by Western blot, MA1-840 detects an unidentified protein at ~30-40 kDa. This antibody does not react with NIH-3T3 cells in Western blot.
The MA1-840 immunogen is a synthetic peptide corresponding to residues 477-947 of human SRC-1. The epitope for MA1-840 has been mapped to the extreme C-terminal end of this fragment.
Target Information
Clone 1135 recognizes SRC-1, steroid-receptor coactivator-1. SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ASV; bHLHe74; c-SRC; Class E basic helix-loop-helix protein 74; Hin-2; Hin-2 protein; mNRC-1; NCoA-1; Nuclear receptor coactivator 1; Nuclear receptor coactivator protein 1; p60-Src; Protein Hin-2; Renal carcinoma antigen NY-REN-52; RIP160; SRC-1; SRC-1 (-Q); SRC-a/NCoA-1; Steroid receptor coactivator 1; steroid receptor coactivator-1; unnamed protein product
Gene Aliases: bHLHe42; BHLHE74; F-SRC-1; KAT13A; NCOA1; RIP160; SRC-1; SRC1
UniProt ID: (Human) Q15788, (Mouse) P70365
Entrez Gene ID: (Human) 8648, (Mouse) 17977
RNA polymerase II regulatory region sequence-specific DNA binding
DNA binding
chromatin binding
transcription coactivator activity
histone acetyltransferase activity
protein binding
transferase activity
transferase activity, transferring acyl groups
ligand-dependent nuclear receptor binding
estrogen receptor binding
retinoic acid receptor binding
macromolecular complex binding
retinoid X receptor binding
protein dimerization activity
peptide-lysine-N-acetyltransferase activity
RNA polymerase II regulatory region DNA binding
signal transducer activity
transcription factor binding
enzyme binding
ligand-dependent nuclear receptor transcription coactivator activity
protein complex binding
progesterone receptor binding
nuclear hormone receptor binding
protein N-terminus binding
histone modifying enzyme
positive regulation of transcription from RNA polymerase II promoter by galactose
regulation of thyroid hormone mediated signaling pathway
chromatin remodeling
regulation of transcription, DNA-templated
lactation
male gonad development
response to hormone
cerebellum development
hippocampus development
hypothalamus development
cerebral cortex development
intracellular estrogen receptor signaling pathway
response to estradiol
response to retinoic acid
response to progesterone
cellular response to hormone stimulus
peroxisome proliferator activated receptor signaling pathway
mRNA transcription from RNA polymerase II promoter
positive regulation of apoptotic process
estrous cycle
positive regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of female receptivity
positive regulation of transcription from RNA polymerase II promoter
progesterone receptor signaling pathway
male mating behavior
labyrinthine layer morphogenesis
regulation of cellular response to insulin stimulus
cellular response to Thyroglobulin triiodothyronine
positive regulation of adipose tissue development
transcription, DNA-templated
signal transduction
histone H4 acetylation
regulation of nucleic acid-templated transcription
regulation of cellular response to drug
regulation of RNA biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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