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Invitrogen
CYP1A1/CYP1A2 Monoclonal Antibody (MC1)
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FIGURE: 1 / 8
CYP1A1/CYP1A2 Antibody (MA3-036) in ICC/IF
Immunofluorescent analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody (Product # MA3-036) in 3% BSA-PBS at a dilution of 1:100 and incu... View More
Product Details
MA3-036
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MC1
Immunogen
3-methylcholanthrene induced rat cytochrome P450 protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-036 detects Cytochrome P450 1A1/1A2 from human, primate, rat and mouse samples.
MA3-036 has been successfully used in Western blot, immunocytochemistry, immunofluorescence and immunohistochemisty (paraffin) applications. By Western blot, this antibody detects a ~58 kDa band representing Cytochrome P450 1A1/1A2.
The MA3-036 immunogen is 3-methylcholanthrene induced rat cytochrome P450 protein.
Target Information
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aromatic compound inducible; aryl hydrocarbon hydroxylase; Cholesterol 25-hydroxylase; CYP1A1; CYP1A1v; alternatively spliced; CYPIA1; CYPIA2; Cytochrome P(3)450; Cytochrome P-448; cytochrome P-450 peptide; cytochrome P-450 protein; cytochrome P-450-1; Cytochrome P-450d; cytochrome P1-450; cytochrome P1-450, dioxin-inducible; Cytochrome P3 450; Cytochrome P450 1A1; Cytochrome P450 1A2; Cytochrome P450 4; cytochrome P450 family 1 subfamily a polypeptide 1; cytochrome P450 family 1 subfamily a polypeptide 2; Cytochrome P450 form 6; cytochrome P450 subfamily I (aromatic compound-inducible) member A1 (C6 form c) (C6 form c); Cytochrome P450 subfamily I (aromatic compound-inducible) member A2 (Q42 form d); cytochrome P450 subfamily I, polypeptide 1; cytochrome P450, 1a1; cytochrome P450, 1a1, aromatic compound inducible; cytochrome P450, 1a2; cytochrome P450, 1a2, aromatic compound inducible; cytochrome P450, family 1, subfamily A, polypeptide 1; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound-inducible), member A1 (C6, form c) (C6, form c); cytochrome P450, subfamily I (aromatic compound-inducible), member A2 (Q42, form d); cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; Cytochrome P450-C; Cytochrome P450-D; Cytochrome P450-P1; Cytochrome P450-P2; Cytochrome P450-P3; Cytochrome P450MT2; dioxin-inducible; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; functional drug metabolizing enzyme; Hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P-448; P450 form 4; P450-D; P450form6; P450MT2; unnamed protein product; xenobiotic monooxygenase
Gene Aliases: AHH; AHRR; CP11; CP12; CYP1; Cyp1a-1; Cyp1a-2; CYP1A1; CYP1A2; Cyp45c; Cypc45c; CYPD45; CYPIA1; CYPIA2; P-450d; P-450MC; P1-450; P3-450; P450(PA); P450-1; P450-3; P450-C; P450DX; RATCYPD45
UniProt ID: (Human) P04798, (Human) P05177, (Mouse) P00184, (Rat) P00185, (Mouse) P00186, (Rat) Q64588
Entrez Gene ID: (Human) 1543, (Human) 1544, (Mouse) 13076, (Rat) 24296, (Mouse) 13077, (Rat) 24297
catalytic activity
monooxygenase activity
iron ion binding
protein binding
arachidonic acid monooxygenase activity
steroid hydroxylase activity
oxidoreductase activity
oxidoreductase activity, acting on diphenols and related substances as donors
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
flavonoid 3'-monooxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
lyase activity
oxygen binding
enzyme binding
heme binding
Hsp70 protein binding
demethylase activity
metal ion binding
Hsp90 protein binding
vitamin D 24-hydroxylase activity
estrogen 16-alpha-hydroxylase activity
estrogen 2-hydroxylase activity
long-chain fatty acid omega-hydroxylase activity
hydroperoxy icosatetraenoate dehydratase activity
long-chain fatty acid omega-1 hydroxylase activity
electron carrier activity
caffeine oxidase activity
aromatase activity
response to hypoxia
long-chain fatty acid metabolic process
liver development
lipid hydroxylation
lipid metabolic process
fatty acid metabolic process
steroid biosynthetic process
steroid catabolic process
porphyrin-containing compound metabolic process
xenobiotic metabolic process
steroid metabolic process
estrogen metabolic process
amine metabolic process
toxin metabolic process
response to xenobiotic stimulus
response to nematode
response to herbicide
response to toxic substance
ethylene metabolic process
coumarin metabolic process
flavonoid metabolic process
response to iron(III) ion
insecticide metabolic process
dibenzo-p-dioxin metabolic process
phenol-containing compound metabolic process
dibenzo-p-dioxin catabolic process
epoxygenase P450 pathway
response to food
response to lipopolysaccharide
response to vitamin A
response to genistein
response to immobilization stress
xenobiotic catabolic process
vitamin D metabolic process
hormone metabolic process
retinol metabolic process
long-chain fatty acid biosynthetic process
9-cis-retinoic acid biosynthetic process
camera-type eye development
nitric oxide metabolic process
response to arsenic-containing substance
digestive tract development
tissue remodeling
hydrogen peroxide biosynthetic process
response to hyperoxia
maternal process involved in parturition
hepatocyte differentiation
cellular response to copper ion
omega-hydroxylase P450 pathway
olefinic compound metabolic process
positive regulation of G1/S transition of mitotic cell cycle
response to diphenyl ether
response to Aroclor 1254
response to 2,3,7,8-tetrachlorodibenzodioxine
response to 3-methylcholanthrene
drug metabolic process
heterocycle metabolic process
oxidation-reduction process
demethylation
cellular response to organic cyclic compound
cellular aromatic compound metabolic process
toxin biosynthetic process
post-embryonic development
alkaloid metabolic process
regulation of gene expression
monoterpenoid metabolic process
lung development
monocarboxylic acid metabolic process
drug catabolic process
exogenous drug catabolic process
cellular respiration
oxidative demethylation
cellular response to cadmium ion
oxidative deethylation
aging
cell proliferation
response to wounding
response to virus
embryo development ending in birth or egg hatching
response to organic substance
response to organic cyclic compound
developmental process
response to drug
response to antibiotic
cholesterol metabolic process
arachidonic acid metabolic process
methylation
aflatoxin metabolic process
response to estradiol
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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