Immunofluorescence analysis of EGFR was done on 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled EGFR (111.6+199.12+ F4+11E8) Mouse Monoclonal Antibody (Product # MA5-13343) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More

EGFR Antibody in Immunoprecipitation (IP)

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EGFR Antibody — EGF Receptor (EGFR) Protein Schematic. EGF Receptor (EGFR) Protein Schematic-Epidermal Growth Factor Receptor, (EGFR, a 185 kDa glycoprotein), also known as erbB, is a transmembrane tyrosine kinase that regulates a variety of biological responses ranging from mitogenesis to stress signaling. The EGFR consists of a large extracellular domain, a single transmembrane domain and a cytoplasmic domain that exhibits kinase activity. Upon binding of EGF to the extracellular domain, the receptor undergoes dimerization and becomes phosphorylated on several tyrosine residues within the cytoplasmic domain. These events result in EGFR activation and increased tyrosine kinase activity toward a variety of intracellular substrates.

Product Details

MA5-13343

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mL

View 3 publications 3 publications

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1/IgG2a

Class

Cocktail

Type

Antibody

Clone

111.6, 199.12, F4, 11E8

Immunogen

Extracellular and cytoplasmic domains of recombinant human EGFR protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

100 µg

Storage conditions

4°C

Shipping conditions

Wet ice

AB_10984241

Product Specific Information

MA5-13343 targets Epidermal Growth Factor Receptor in IP and WB applications and shows reactivity with Human samples.

The MA5-13343 immunogen is extracellular and cytoplasmic domains of recombinant human EGFR protein.

This cocktail is also referred to as clone R19/48.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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EGFR Antibody Cocktail

EGFR Antibody (MA5-13343) in ICC/IF