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Invitrogen
Alpha-Smooth Muscle Actin Monoclonal Antibody (4A4)
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FIGURE: 1 / 9
Alpha-Smooth Muscle Actin Antibody (MA5-15871) in ICC/IF
Immunofluorescence analysis of Alpha Smooth Muscle Actin was performed using 70% confluent log phase C2C12 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Alpha-Smooth Muscle Actin Monoclonal Antibody (4A4) (Product # MA5-15871) at 1:200 dilution ... View More
Product Details
MA5-15871
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4A4
Immunogen
Synthesized peptide of human ACTA2.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15871 targets ACTA2 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
The MA5-15871 immunogen is synthesized peptide of human ACTA2. .
MA5-15871 detects ACTA2 which has a predicted molecular weight of approximately 42kDa.
Target Information
Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ACTA2; Actin Alpha 2 Smooth Muscle Aorta; Actin Vascular Smooth Muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Alpha Cardiac Actin; alpha-actin; Alpha-actin-2; alpha-cardiac actin; Aortic Smooth Muscle; asma; Cell growth-inhibiting gene 46 protein; GIG46; Growth Inhibiting Gene 46; smooth muscle; smooth muscle alpha-actin; unnamed protein product
Gene Aliases: 0610041G09Rik; a-SMA; ACTA2; ACTSA; ACTVS; alphaSMA; GIG46; SMalphaA; SMDYS
UniProt ID: (Human) P62736, (Rat) P62738, (Mouse) P62737
Entrez Gene ID: (Human) 59, (Rat) 81633, (Mouse) 11475
muscle contraction
regulation of blood pressure
response to virus
positive regulation of gene expression
vascular smooth muscle contraction
positive regulation of hepatic stellate cell migration
positive regulation of hepatic stellate cell contraction
positive regulation of ERK1 and ERK2 cascade
cellular response to transforming growth factor beta stimulus
juxtaglomerular apparatus development
glomerular mesangial cell development
mesenchyme migration
positive regulation of hepatic stellate cell activation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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