Immunocytochemistry analysis of CADM1 in SH-SY-5Y cells. Samples were incubated with CADM1 polyclonal antibody (Product # PA3-16744) followed by Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

CADM1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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CADM1 Antibody — Sub-saturating lentiviral artificial microRNA (amiRNA) transduction potently knocks down synaptic cell adhesion molecules 1-3 (SynCAM1-3) in amiRNA+ cells while keeping a subpopulation of wild type (WT) amiRNA- cells. (A) Lentiviral knockdown (KD) vectors were generated with a minimal CMV (mCMV) promoter, intronic amiRNAs, and nlsGFP. RSV, Rous sarcoma virus promoter; LTR, long-terminal repeat; RRE, Rev-response element; cPPT, central polypurine tract; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element; SA, splice acceptor; SD, splice donor. (B) memGFP-expressing lentiviral vector for dendrite labeling. (C) Comparison of cadm1-3 mRNA levels at 14-15 days in vitro (DIV) by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in cultured rat hippocampal neurons using saturating viral titers relative to levels in uninfected control cultures. n = 2 experiments. (D) Transduction rate of cultured neurons at 14-15 DIV using sub-saturating viral titers. n = 2 experiments. (E) Representative blots and (F) relative SynCAM1-3 protein levels at 13-15 DIV measured by quantitative western blotting of cultured neurons following indicated amiRNA lentiviral treatments at saturating or sub-saturating viral titers compared to Scrambled1-3 amiRNA control treatments. Actin was used as a loading control. n = 4 (saturating) or n = 2 (sub-saturating) experiments. A single gel image of the same intensity was cropped as marked by dotted lines and rearranged for pr {KD}

Immunogen image

Immunogen Image CADM1 Polyclonal Antibody

Product Details

PA3-16744

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:2,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to the C-terminal region of rat SynCAM/CADM1.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2121871

Product Specific Information

Suggested positive control: rat brain.

Target Information

Mediates homophilic cell-cell adhesion in a Ca (2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca (2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cell adhesion molecule 1; IGSF4; Immunoglobulin superfamily member 4; immunoglobulin superfamily membrane protein; immunoglobulin superfamily, member 4A; NECL-2; nectin-like 2; Nectin-like protein 2; secretory isoform of TSLC1; SgIgSF; Spermatogenic immunoglobulin superfamily; Synaptic cell adhesion molecule; SynCAM; TSLC-1; TSLC1/Nectin-like 2/IGSF4; tumor suppressor; Tumor suppressor in lung cancer 1; Tumor suppressor in lung cancer 1 homolog; unnamed protein product

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Gene Aliases: BL2; CADM1; IGSF4; IGSF4A; Necl-2; NECL2; RA175; sgIGSF; ST17; sTSLC-1; SYNCAM; SynCam1; TSLC1

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UniProt ID: (Human) Q9BY67, (Rat) Q6AYP5

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Entrez Gene ID: (Human) 23705, (Rat) 363058

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Function(s)

receptor binding protein binding PDZ domain binding protein homodimerization activity cell adhesion molecule binding

Process(es)

positive regulation of cytokine production T cell mediated cytotoxicity immune system process apoptotic process cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules spermatogenesis cell recognition cell differentiation susceptibility to natural killer cell mediated cytotoxicity positive regulation of natural killer cell mediated cytotoxicity activated T cell proliferation detection of stimulus liver development synapse assembly brain development unidimensional cell growth calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules positive regulation of cytokine secretion bone development

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CADM1 Polyclonal Antibody

CADM1 Antibody (PA3-16744) in ICC/IF

Product Details

PA3-16744

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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