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Invitrogen
CAF1 p150 Polyclonal Antibody
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FIGURE: 1 / 3
CAF1 p150 Antibody (PA5-116964) in WB
Western blot was performed using CAF1 p150 Polyclonal Antibody (Product # PA5-116964) and a 150 kDa band corresponding to CAF1 p150 was observed and cell cycle dependent variation in expression was observed in HeLa cells treated with multiple treatments. Nuclear enriched extracts (30 µg lysate) of K-562 (Lane 1), A-431 (Lane 2), Hep G2 (Lane 3), SiHa (Lane 4), HeLa (Lane 5), HeLa (G1 phase) 25h block in 2mm thymidine; 12h release in 30 µm 2-deoxycytidine, 25h block in 2mm thymidine followed by 14h release in 30 µm 2-deoxycytidine (Lane 6),... View More
Product Details
PA5-116964
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human p150 CAF1 (NP_0054742)
Conjugate
Form
Liquid
Concentration
0.98 mg/mL
Amount
98 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: HeLa, K-562
Immunogen sequence: MLEELECGAP GARGAATAMD CKDRPAFPVK KLIQARLPFK RLNLVPKGKA DDMSDDQGTS VQSKSPDLEA SLDTLENNCH VGSDIDFRPK LVNGKGPLDN FLRNRIETSI GQSTVIIDLT EDSNEQPDSL VDHNKLNSEA SPSREAINGQ REDTGDQQGL LKAIQNDKLA FPGETLSDIP CKTEEEGVGC GGAGRRGDSQ
Target Information
CAF1 p150 is part of the chromatin assembly factor 1 (CAF-1) complex, a key player in DNA repair and replication processes. CAF-1, composed of p150 (CHAF1A), p60 (CHAF1B), and p48 subunits, functions primarily in the deposition of newly synthesized histones H3 and H4 onto nascent DNA strands, ensuring chromatin is properly assembled during DNA replication and after repair. The p150 subunit is the largest of the CAF-1 complex and is critical for binding DNA and recruiting CAF-1 to replication sites, facilitating histone deposition in a process vital for maintaining genome stability and epigenetic inheritance. Beyond just chromatin formation, CAF1 p150 is involved in influencing gene silencing and the maintenance of heterochromatin, impacting gene regulation notably during development and in response to DNA damage. Its role has implications in oncogenesis, where dysregulation of CAF1 p150 can lead to altered chromatin structures and contribute to tumorigenesis, marking it as an important target in cancer research.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; Chromatin assembly factor 1 subunit A; chromatin assembly factor I (150 kDa); Chromatin assembly factor I p150 subunit; CTB-50L17.7; hp150; MGC71229
Gene Aliases: CAF; CAF-1; CAF1; CAF1B; CAF1P150; CHAF1A; P150
UniProt ID: (Human) Q13111
Entrez Gene ID: (Human) 10036
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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