Immunofluorescence analysis of Tubulin alpha was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Tubulin alpha Rabbit Polyclonal Antibody (Product # PA5-16891) at 2µg/mL in 0.1% BSA and incubated for 3 hour... View More

alpha Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Tubulin Antibody in Western Blot (WB)

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Immunogen image

Immunogen Image alpha Tubulin Polyclonal Antibody

Product Details

PA5-16891

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

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Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

2 µg/mL

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Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide derived from aa 426-450 of human tubulin alpha

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10984790

Product Specific Information

PA5-16891 targets Tubulin alpha in immunohistochemistry (paraffin) and Western blot applications and shows reactivity with Bovine, Chicken, amphibian, Gerbil, Guinea Pig, Human, mouse, Porcine, and Rat samples.

The PA5-16891 immunogen is a synthetic peptide derived from aa 426-450 of human tubulin alpha.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha tubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; epididymis secretory sperm binding protein; FLJ30169; gangliocyte protein related to virus stimulation; H2 ALPHA; hum-a-tub1; hum-a-tub2; MGC8685; similar to beta tubulin; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; Tubulin alpha chain-like 3; tubulin alpha-1; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 2; tubulin, alpha 4; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, beta 2; tubulin, beta 2B; tubulin, beta polypeptide paralog; unnamed protein product

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Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; CDCBM7; CMYO26; FTDALS9; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; OZEMA23; OZEMA24; PMGYSA; RGD1309427; RGD1565476; SPAX11; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; Tubb2; Tubb2a; TUBB2B

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Function(s)

nucleotide binding structural molecule activity structural constituent of cytoskeleton protein binding GTP binding hydrolase activity identical protein binding macromolecular complex binding metal ion binding protein heterodimerization activity double-stranded RNA binding GTPase activity ubiquitin protein ligase binding enzyme binding protein kinase binding protein domain specific binding molecular_function tubulin

Process(es)

microtubule cytoskeleton organization mitotic cell cycle neuron migration startle response intracellular protein transport microtubule-based process centrosome cycle smoothened signaling pathway memory locomotory behavior adult locomotory behavior visual learning response to mechanical stimulus glial cell differentiation gene expression dentate gyrus development cerebellar cortex morphogenesis hippocampus development pyramidal neuron differentiation cerebral cortex development neurogenesis neuron differentiation sperm motility adult behavior cytoskeleton-dependent intracellular transport response to tumor necrosis factor locomotory exploration behavior microtubule polymerization forebrain morphogenesis homeostasis of number of cells within a tissue regulation of synapse organization synapse organization cell division neuron apoptotic process motor behavior cellular response to calcium ion organelle transport along microtubule neuron projection arborization response to L-glutamate cellular response to interleukin-4 cytoskeleton organization nervous system development modulation of synaptic transmission positive regulation of axon guidance embryonic brain development protein polymerization metabolic process biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Tubulin Polyclonal Antibody

alpha Tubulin Antibody (PA5-16891) in ICC/IF