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Invitrogen
PHOX2B Polyclonal Antibody
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FIGURE: 1 / 2
PHOX2B Antibody (PA5-29025) in ICC/IF
PHOX2B Polyclonal Antibody detects PHOX2B protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHOX2B protein stained by PHOX2B Polyclonal Antibody (Product # PA5-29025) diluted at 1:1,000. Blue: Hoechst 33342 staining.
Product Details
PA5-29025
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PHOX2B. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLaS3.
Predicted reactivity: Mouse (100%), Xenopus laevis (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NBPhox; neuroblastoma paired-type homeobox protein; Neuroblastoma Phox; paired like homeobox2B; paired mesoderm homeobox 2b; Paired mesoderm homeobox protein 2B; Paired-like homeobox 2B; PHOX2B homeodomain protein
Gene Aliases: CCHS; NBLST2; NBPhox; PHOX2B; PMX2B
UniProt ID: (Human) Q99453
Entrez Gene ID: (Human) 8929
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
sequence-specific double-stranded DNA binding
neuron migration
regulation of respiratory gaseous exchange by neurological system process
noradrenergic neuron differentiation
noradrenergic neuron development
brainstem development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cell proliferation
glial cell differentiation
regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
response to activity
cell differentiation in hindbrain
medullary reticular formation development
hindbrain tangential cell migration
neuron differentiation
skeletal muscle cell differentiation
type B pancreatic cell proliferation
negative regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
cell development
autonomic nervous system development
enteric nervous system development
sympathetic nervous system development
parasympathetic nervous system development
neuron development
inner ear development
efferent axon development in a lateral line nerve
membrane depolarization
respiratory system development
retrotrapezoid nucleus neuron differentiation
sympathetic ganglion development
cellular response to carbon dioxide
dopaminergic neuron differentiation
cellular response to BMP stimulus
motor neuron migration
positive regulation of cold-induced thermogenesis
neural crest cell migration involved in autonomic nervous system development
negative regulation of type B pancreatic cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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