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Invitrogen
Influenza A PA Polyclonal Antibody
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FIGURE: 1 / 4
Influenza A PA Antibody (PA5-32223) in ICC/IF
Influenza A Virus PA antibody detects PA protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: PA protein stained by Influenza A Virus PA antibody (Product # PA5-32223) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C,30 min.
Product Details
PA5-32223
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Published species
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PA (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Amount
30 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Influenza viruses consists of three types (Influenza A, B, and C), which make up three of the five genera in the Orthomyxoviridae family. Commonly know as the flu, influenza strains can affect both birds and mammals. Influenza strains form roughly spherical particles and are similar in composition with viral envelope containing two main types of glycoproteins (hemagglutinin and neuraminidase), wrapped around the central core of the viral RNA genome and other viral proteins that package and protect the RNA. Unusually for a virus, the influenzavirus genome is not a single piece of nucleic acid; rather a seven to eight segmented fragments of negative-sense RNA with each fragment expressing different viral proteins. Influenza spreads seasonally, resulting in about three to five million yearly cases of severe illness and about 250,000 to 500,000 deaths annually or into the millions in pandemic years. Influenza A virus contains eight single stranded, negative sense RNAs. Each RNA is associated with viral RNA-dependent RNA polymerase which consists of PA, PB1 and PB2 proteins. Recent reports indicate the involvement of PA in 'cap-snatching' mechanism via its endonuclease activity which is essential for cRNA-dependent viral RNA synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Influenza PA
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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