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Invitrogen
CD49a Polyclonal Antibody
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FIGURE: 1 / 8
CD49a Antibody (PA5-47763) in ICC/IF
Immunofluorescence analysis of CD49a was performed using 70 % confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD49a Polyclonal Antibody (Product # PA5-47763) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A11015),... View More
Product Details
PA5-47763
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CHO-derived recombinant human Integrin alpha 1/CD49a Phe29-Pro1141
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin alpha 2, rhIntegrin alpha 11, recombinant mouse (rm) Integrin alpha 2, and rmIntegrin alpha 11 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD49 antigen-like family member A; CD49a; Integrin alpha-1; Laminin and collagen receptor; unnamed protein product; very late activation protein 1; very late antigen-1; VLA-1
Gene Aliases: CD49a; ITGA1; VLA1
UniProt ID: (Human) P56199
Entrez Gene ID: (Human) 3672
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
negative regulation of cell proliferation
neutrophil chemotaxis
negative regulation of epidermal growth factor receptor signaling pathway
vasodilation
positive regulation of MAPK cascade
positive regulation of neuron apoptotic process
cellular extravasation
neuron projection morphogenesis
cell chemotaxis
cell-cell adhesion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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