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Invitrogen
Cyclin B1 Polyclonal Antibody
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FIGURE: 1 / 3
Cyclin B1 Antibody (PA5-78941) in ICC/IF
Immunocytochemistry analysis of CCNB1 using anti-CCNB1 antibody (Product # PA5-78941) . CCNB1 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-CCNB1 antibody (Product # PA5-78941) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit... View More
Product Details
PA5-78941
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: HELA whole cell, 293T whole cell, MCF-7 whole cell, COLO320 whole cell. ICC/IF: A431 cell. Flow: THP-1 cell.
Target Information
Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G2/mitotic-specific cyclin-B1; OTTHUMP00000221981; OTTHUMP00000223023; unnamed protein product
Gene Aliases: CCNB; CCNB1
UniProt ID: (Human) P14635
Entrez Gene ID: (Human) 891
G1/S transition of mitotic cell cycle
G2/M transition of mitotic cell cycle
oocyte maturation
in utero embryonic development
mitotic spindle organization
mitotic metaphase plate congression
spermatogenesis
response to xenobiotic stimulus
response to mechanical stimulus
response to toxic substance
negative regulation of gene expression
positive regulation of G2/M transition of mitotic cell cycle
positive regulation of mRNA 3'-end processing
tissue regeneration
mitotic cell cycle phase transition
positive regulation of cell cycle
positive regulation of mitotic cell cycle
response to DDT
positive regulation of fibroblast proliferation
digestive tract development
cell division
positive regulation of attachment of spindle microtubules to kinetochore
ventricular cardiac muscle cell development
positive regulation of cardiac muscle cell proliferation
regulation of chromosome condensation
protein-containing complex assembly
cellular response to iron(III) ion
cellular response to fatty acid
cellular response to hypoxia
regulation of mitotic cell cycle spindle assembly checkpoint
positive regulation of mitochondrial ATP synthesis coupled electron transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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