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Invitrogen
MCM2 Polyclonal Antibody
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FIGURE: 1 / 12
MCM2 Antibody (PA5-79645) in ICC/IF
Immunocytochemistry analysis of MCM2 using anti-MCM2 antibody (Product # PA5-79645) . MCM2 was detected in a section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-MCM2 antibody (Product # PA5-79645) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Vi... View More
Product Details
PA5-79645
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse MCM2 (884-904aa DLFKFNKFSRDLKRKLILQQF).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: Rat Testis Tissue, Rat Kidney Tissue, Mouse Intestine Tissue, Mouse Testis Tissue, Mouse Ovary Tissue, Mouse Kidney Tissue. IHC: Rat Intestinal Lymphocyte Tissue, Mouse Intestine Tissue. ICC/IF: HEPA1-6 cell, HEPA Cell, NIH3T3 Cell. Flow: Neuro-2a cell.
Target Information
MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. MCM2 has been mapped to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cell devision cycle-like 1; cyclin-like 1; DNA replication licensing factor MCM2; KIAA0030; MGC10606; mini chromosome maintenance deficient 2; minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28; unnamed protein product
Gene Aliases: AA959861; AW476101; BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; KIAA0030; MCM2; Mcmd2; MITOTIN; mKIAA0030
UniProt ID: (Human) P49736, (Mouse) P97310
Entrez Gene ID: (Human) 4171, (Rat) 312538, (Mouse) 17216
nucleotide binding
DNA binding
DNA helicase activity
DNA replication origin binding
single-stranded DNA binding
helicase activity
protein binding
ATP binding
zinc ion binding
hydrolase activity
ATPase activity
single-stranded DNA-dependent ATP-dependent DNA helicase activity
enzyme binding
histone binding
3'-5' DNA helicase activity
metal ion binding
DNA metabolism protein
double-strand break repair via break-induced replication
DNA replication
DNA replication initiation
nucleosome assembly
apoptotic process
regulation of DNA-dependent DNA replication initiation
cellular response to interleukin-4
cochlea development
mitotic DNA replication initiation
DNA unwinding involved in DNA replication
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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