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Invitrogen
RFFL Polyclonal Antibody
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FIGURE: 1 / 1
RFFL Antibody (PA5-93015) in WB
Western blot analysis of RFFL in extracts of U-251MG cells. Samples were incubated with RFFL Polyclonal antibody (Product # PA5-93015) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
Product Details
PA5-93015
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human RFFL (NP_0010173681)
Conjugate
Form
Liquid
Concentration
0.15 mg/mL
Amount
15 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MWATCCNWFC LDGQPEEVPP PQGARMQAYS NPGYSSFPSP TGLEPSCKSC GAHFANTARK QTCLDCKKNF CMTCSSQVGN GPRLCLLCQR FRATAFQREE LMKMKVKDLR DYLSLHDIST EMCREKEELV LLVLGQQPVI SQEDRTRAST LSPDFPEQQA FLTQPHSSMV PPTSPNLPSS SAQATSVPPA; Positive Samples: U-251MG; Cellular Location: Cell membrane, Cytoplasm, Peripheral membrane protein, Recycling endosome membrane, cytosol
Target Information
Has E3 ubiquitin protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity. May bind phosphatidylinositol phosphates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CARP-2; caspase 8 and 10 associated RING protein-2; Caspase regulator CARP2; Caspases-8 and -10-associated RING finger protein 2; E3 ubiquitin-protein ligase rififylin; Fring; FYVE-RING finger protein SAKURA; rififylin; ring finger and FYVE like domain containing protein; ring finger and FYVE-like domain containing 1; RING finger and FYVE-like domain-containing protein 1; RING finger protein 189; RING finger protein 34-like; RING finger protein SAKURA; RING-type E3 ubiquitin transferase rififylin; unnamed protein product
Gene Aliases: 1700051E09Rik; 4930516L10Rik; BG080975; CARP-2; CARP2; FRING; RFFL; RIFIFYLIN; RNF189; RNF34L
UniProt ID: (Human) Q8WZ73, (Mouse) Q6ZQM0, (Rat) Q8CIN9
Entrez Gene ID: (Human) 117584, (Mouse) 67338, (Rat) 282844
protease binding
p53 binding
protein binding
zinc ion binding
transferase activity
protein kinase binding
ubiquitin protein ligase binding
metal ion binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
molecular_function
ubiquitin-protein ligase
ubiquitin-dependent protein catabolic process
intracellular protein transport
apoptotic process
regulation of fibroblast migration
negative regulation of tumor necrosis factor-mediated signaling pathway
protein ubiquitination
regulation of TOR signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
protein K48-linked ubiquitination
regulation of signal transduction by p53 class mediator
negative regulation of signal transduction by p53 class mediator
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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