C_990000001_10 targets a gender-specific polymorphic region in the amelogenin gene that is commonly used in forensic sex determination tests. A 6 nt deletion occurs in the X-specific amelogenin gene (B37/hg19 genome locations: chrX:11315039 and chrY:6737949-6737954). The VIC dye probe detects X-chromosome sequences and the FAM dye probe detects Y-chromosome sequences; male samples will run in the heterozygous cluster position. Some males lack the Y-specific amelogenin gene and will type as female, thus C_990000001_10 should be run in combination with a Y-chromosome assay to identify any mistyped individuals. For more information, go to:http://www.cstl.nist.gov/strbase/Amelogenin.htm