Analytical sensitivity
0.01 ng/mL
Assay range
0.08-5.0 ng/mL
Sample type/volume
Plasma
50 µL
Serum
20 µL
Supernatant
50 µL
Hands-on time
1 hr 20 min
Time-to-result
3 hr 10 min
Homogenous (no wash)
No
Interassay CV
6.6%
Intraassay CV
5.5%
Instrument
Colorimetric Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plates
Standard
Sample Diluent
Assay Buffer concentrate
HRP-conjugated Detection Antibody
Wash Buffer
Controls
Chromogen
Stop Solution
Adhesive Plate Covers
Storage
2-8°C
Protein name
CD154 (CD40 Ligand)
Target sub-specificity
Soluble
Protein aliases
CD154, CD40 antigen ligand, CD40 ligand, membrane form, soluble form, CD40-L, sCD40L, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein, TRAP, tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5
Species (tested)
Human
Assay kit format
High Sensitivity Sandwich ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
Gene aliases
CD154, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP
Gene ID
Gene symbol
CD40LG
UniProt ID
The Human CD40 Ligand (CD40L) ELISA quantitates Hu CD40L in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD40L.
Principle of the method
The Human CD40L US solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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