GeneArt™ Gibson Assembly HiFi Master Mix, 10 reactions - FAQs

Q: What type of inserts can I use with Gibson Assembly kits?

Any linearized DNA fragment, including PCR products, restriction digestion products, and GeneArt Strings can be used with Gibson Assembly kits.

Q: What is the difference between the Gibson Assembly HiFi and the Gibson Assembly EX kits?

Gibson Assembly HiFi kits provide the quickest and easiest way to assemble up to six fragments in a single-tube reaction. The more robust Gibson Assembly EX kits enable assembly of up to 15 fragments in a single-tube, two-step reaction.

View additional product information for GeneArt™ Gibson Assembly HiFi Master Mix - FAQs (A46628, A46627, A46629)

4 product FAQs found

How big do DNA fragment homology arms need to be when using Gibson Assembly kits?

For fragments that are 8 kb or lesser in length, an overlap of 20-40 bp is recommended. For fragments greater than 8 kb in length, an overlap of 40-100 bp is recommended.

What type of vector can I use with Gibson Assembly kits?

Any linearized vector can be used with Gibson Assembly kits. We recommend using high-copy vectors for small inserts (<10 kb) and low-copy vectors for large inserts or toxic genes.

What type of inserts can I use with Gibson Assembly kits?

Any linearized DNA fragment, including PCR products, restriction digestion products, and GeneArt Strings can be used with Gibson Assembly kits.

What is the difference between the Gibson Assembly HiFi and the Gibson Assembly EX kits?

Gibson Assembly HiFi kits provide the quickest and easiest way to assemble up to six fragments in a single-tube reaction. The more robust Gibson Assembly EX kits enable assembly of up to 15 fragments in a single-tube, two-step reaction.