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Invitrogen

CX3CR1 Monoclonal Antibody (2A9-1), APC, eBioscience™

3 Published Figures
5 References
View all (35) CX3CR1 antibodies

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Product Details

17-6099-41

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
View 5 publications 5 publications
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2A9-1

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11148939

Product Specific Information

Description: This 2A9-1 monoclonal antibody reacts with human CX3CR1, which is the receptor for fractalkine, a transmembrane chemokine of the CX3C family. CX3CR1 is a seven transmembrane G protein-coupled receptor expressed on CD16+ NK cells, T cells (e.g. CD8+, CD4+, and gamma/delta), and monocytes. In non-immune cells, CX3CR1 has been found on osteoclast precursors and microglia. Little to no CX3CR1 surface expression can be detected on B cells and granulocytes. Together, fractalkine and its receptor mediate cell-cell adhesion and chemotaxis of NK cells, T cells, and monocytes. The expression of CX3CR1 has also been correlated with high levels of intracellular perforin and granzyme B.

Applications Reported: This 2A9-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2A9-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration an d adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: Beta chemokine receptor-like 1; C-X3-C CKR-1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMK-BRL-1; CMK-BRL1; Cx3c; CX3C chemokine receptor 1; Fractalkine receptor; G-protein coupled receptor 13; Scyd1; unnamed protein product; V28

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Gene Aliases: CCRL1; CMKBRL1; CMKDR1; CX3CR1; GPR13; GPRV28; V28

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UniProt ID: (Human) P49238

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Entrez Gene ID: (Human) 1524

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Function(s)
cytokine receptor activity G-protein coupled receptor activity chemokine receptor activity protein binding G-protein coupled peptide receptor activity C-C chemokine receptor activity C-X3-C chemokine receptor activity C-C chemokine binding C-X3-C chemokine binding CX3C chemokine receptor binding
Process(es)
positive regulation of neuroblast proliferation adaptive immune response microglial cell activation involved in immune response immune system process response to ischemia chemotaxis inflammatory response immune response cellular defense response cell adhesion signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration cell-cell signaling brain development response to wounding negative regulation of angiogenesis cytokine-mediated signaling pathway calcium-mediated signaling central nervous system maturation leukocyte chemotaxis regulation of tumor necrosis factor production negative regulation of interleukin-1 beta production social behavior autocrine signaling positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response regulation of nitric oxide biosynthetic process regulation of synaptic plasticity host-mediated regulation of intestinal microbiota composition regulation of neurogenesis positive regulation of neurogenesis modulation of synaptic transmission leukocyte tethering or rolling positive regulation of protein kinase B signaling synapse maturation cell chemotaxis antifungal innate immune response chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to cytokine stimulus positive regulation of monocyte chemotaxis synapse pruning negative regulation of hippocampal neuron apoptotic process multiple spine synapse organization, single dendrite regulation of microglial cell migration negative regulation of microglial cell mediated cytotoxicity negative regulation of apoptotic signaling pathway
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