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OriGene
PRKCE Monoclonal Antibody (OTI1B4), TrueMAB™
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Product Details
CF502417
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI1B4
Immunogen
Full length human recombinant protein of human PRKCE produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC125656; MGC125657; nPKC-epsilon; PKC e; Protein kinase C epsilon type; unnamed protein product
Gene Aliases: nPKC-epsilon; PKCE; PRKCE
UniProt ID: (Human) Q02156
Entrez Gene ID: (Dog) 609934, (Human) 5581
nucleotide binding
actin monomer binding
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
calcium-independent protein kinase C activity
protein binding
ATP binding
enzyme activator activity
zinc ion binding
kinase activity
transferase activity
enzyme binding
receptor activator activity
ethanol binding
metal ion binding
14-3-3 protein binding
protein serine kinase activity
non-receptor serine/threonine protein kinase
MAPK cascade
macrophage activation involved in immune response
immune system process
apoptotic process
cell adhesion
signal transduction
toxin catabolic process
positive regulation of epithelial cell migration
positive regulation of fibroblast migration
positive regulation of cell-substrate adhesion
regulation of metal ion transport
peptidyl-serine phosphorylation
regulation of lipid metabolic process
insulin secretion
positive regulation of actin filament polymerization
negative regulation of protein ubiquitination
cell-substrate adhesion
lipopolysaccharide-mediated signaling pathway
positive regulation of insulin secretion
positive regulation of synaptic transmission, GABAergic
positive regulation of cytokinesis
positive regulation of superoxide anion generation
intracellular signal transduction
locomotory exploration behavior
TRAM-dependent toll-like receptor 4 signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
xenobiotic catabolic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to morphine
positive regulation of MAPK cascade
positive regulation of lipid catabolic process
regulation of release of sequestered calcium ion into cytosol
cell division
establishment of localization in cell
synaptic transmission, GABAergic
regulation of insulin secretion involved in cellular response to glucose stimulus
mucus secretion
positive regulation of mucus secretion
cellular response to ethanol
cellular response to prostaglandin E stimulus
cellular response to hypoxia
positive regulation of wound healing
negative regulation of sodium ion transmembrane transport
positive regulation of protein localization to plasma membrane
regulation of cation transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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