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Syk Antibody (MA1-19332) in ICC/IF
Immunofluorescence of SYK was performed using THP-1 wild-type and SYK KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) or methanol for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the SYK monoclonal antibody (P... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-19332
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
SYK-01
Immunogen
Recombinant fragment (aa 5-360) of human Syk.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with protein tyrosine kinase p72Syk (Syk; an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).
Target Information
Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that are viewed as potential druge targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp313N1010; EC 2.7.10.2; FLJ25043; FLJ37489; HGNC:11205; kinase Syk; KSYK; SP1; spleen tyrosine kinase; syk signaling; tyrosine-kinase syk; unnamed protein product
Gene Aliases: IMD82; p72-Syk; p72syk; Sykb
UniProt ID: (Human) P43405, (Mouse) P48025, (Rat) Q64725
Entrez Gene ID: (Human) 6850, (Mouse) 20963, (Rat) 25155
phosphotyrosine binding
protein kinase activity
protein serine/threonine kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
receptor binding
integrin binding
protein binding
ATP binding
interleukin-15 receptor binding
kinase activity
protein kinase binding
phosphatase binding
Toll-like receptor binding
SH2 domain binding
phospholipase binding
scaffold protein binding
protein domain specific binding
ubiquitin protein ligase binding
immunoglobulin receptor binding
cell activation
lymph vessel development
positive regulation of receptor internalization
stimulatory C-type lectin receptor signaling pathway
adaptive immune response
macrophage activation involved in immune response
neutrophil activation involved in immune response
leukocyte activation involved in immune response
serotonin secretion by platelet
cell surface pattern recognition receptor signaling pathway
negative regulation of inflammatory response to antigenic stimulus
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
protein import into nucleus
leukocyte cell-cell adhesion
integrin-mediated signaling pathway
organ morphogenesis
regulation of platelet activation
regulation of tumor necrosis factor-mediated signaling pathway
peptidyl-tyrosine phosphorylation
platelet activation
neutrophil chemotaxis
positive regulation of protein complex assembly
receptor internalization
positive regulation of type I interferon production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-4 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
regulation of superoxide anion generation
positive regulation of superoxide anion generation
positive regulation of cell adhesion mediated by integrin
positive regulation of toll-like receptor 9 signaling pathway
intracellular signal transduction
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
interleukin-3-mediated signaling pathway
defense response to bacterium
regulation of neutrophil degranulation
innate immune response
positive regulation of B cell differentiation
positive regulation of lymphocyte differentiation
positive regulation of bone resorption
blood vessel morphogenesis
positive regulation of inflammatory response
regulation of phagocytosis
regulation of immune response
B cell receptor signaling pathway
leukocyte migration
regulation of ERK1 and ERK2 cascade
cellular response to molecule of fungal origin
cellular response to lipid
cellular response to low-density lipoprotein particle stimulus
positive regulation of monocyte chemotactic protein-1 production
regulation of arachidonic acid secretion
regulation of platelet aggregation
apoptotic signaling pathway
beta-amyloid clearance
positive regulation of cold-induced thermogenesis
positive regulation of cGAS/STING signaling pathway
positive regulation of TORC1 signaling
cellular response to beta-amyloid
cellular response to lectin
autophagosome assembly
positive regulation of cytokine production
serotonin secretion
cell surface receptor signaling pathway
enzyme linked receptor protein signaling pathway
G-protein coupled receptor signaling pathway
negative regulation of autophagy
positive regulation of autophagy
peptidyl-serine phosphorylation
leukotriene biosynthetic process
cellular response to nutrient levels
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interleukin-3 production
collagen-activated tyrosine kinase receptor signaling pathway
TORC1 signaling
positive regulation of mast cell degranulation
beta selection
positive regulation of MAPK cascade
positive regulation of JUN kinase activity
positive regulation of gamma-delta T cell differentiation
positive regulation of alpha-beta T cell differentiation
positive regulation of alpha-beta T cell proliferation
protein autophosphorylation
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of calcium-mediated signaling
antigen receptor-mediated signaling pathway
negative regulation of TORC1 signaling
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