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Invitrogen
ITGB1 Monoclonal Antibody (4B7R), FITC
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Product Details
MA1-82001
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4B7R
Immunogen
Ocular melanoma cell line V+B2.
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human CD29 monoclonal antibody, clone 4B7R recognizes the human integrin beta 1 subunit, also known as CD29, a approximately 130 kDa (red) 115 kDa (non-red) single pass type I transmembrane glycoprotein expressed by most leucocytes and mesenchymal stem cells.
Target Information
CD29, also known as ITGB1 or Integrin Subunit Beta 1, is a 110 kDa cell surface glycoprotein widely expressed by various cells, including all leukocytes. It forms non-covalently linked heterodimers with at least six different alpha integrins (CD49a-f), creating VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers mediate a range of cellular responses, including adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins bind to extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin. Some integrins also interact with cellular receptors like VCAM-1 and MadCAM-1. CD29 plays a crucial role in immune cell trafficking from blood to tissue, particularly during leukocyte emigration and extravascular migration. It is involved in regulating tissue damage and disease symptoms related to inflammatory bowel disease. In the presence of anti-tumor drugs, decreased expression of ITGB1 correlates with multidrug resistance in tumor cells. ITGB1-dependent mechanisms enhance cytokine secretion in human airway smooth muscle in response to IL-1beta, facilitated by fibronectin and type I collagen. More than eight beta subunits with numerous splice variant isoforms have been identified in mammals, including two major forms: beta1A and beta1D. Beta1A is present in all tissues except cardiac and skeletal muscle, which express the beta1D variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD29; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; Integrin beta-1; Integrin beta1; ITBG1D; VLA-4 subunit beta
Gene Aliases: FNRB; ITGB1; MDF2; MSK12
Entrez Gene ID: (Human) 3688
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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