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Invitrogen
ITGB1 Monoclonal Antibody (4B7R), PE
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Product Details
MA1-82003
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4B7R
Immunogen
Ocular melanoma cell line V+B2.
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human CD29 monoclonal antibody, clone 4B7R recognizes the human integrin beta 1 subunit, also known as CD29, a approximately 130 kDa (red) 115 kDa (non-red) single pass type I transmembrane glycoprotein expressed by most leucocytes and mesenchymal stem cells.
Target Information
CD29, also known as ITGB1 or Integrin Subunit Beta 1, is a 110 kDa cell surface glycoprotein widely expressed by various cells, including all leukocytes. It forms non-covalently linked heterodimers with at least six different alpha integrins (CD49a-f), creating VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers mediate a range of cellular responses, including adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins bind to extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin. Some integrins also interact with cellular receptors like VCAM-1 and MadCAM-1. CD29 plays a crucial role in immune cell trafficking from blood to tissue, particularly during leukocyte emigration and extravascular migration. It is involved in regulating tissue damage and disease symptoms related to inflammatory bowel disease. In the presence of anti-tumor drugs, decreased expression of ITGB1 correlates with multidrug resistance in tumor cells. ITGB1-dependent mechanisms enhance cytokine secretion in human airway smooth muscle in response to IL-1beta, facilitated by fibronectin and type I collagen. More than eight beta subunits with numerous splice variant isoforms have been identified in mammals, including two major forms: beta1A and beta1D. Beta1A is present in all tissues except cardiac and skeletal muscle, which express the beta1D variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glycoprotein IIa; integrin beta 1; integrin beta-1 subunit (cytoplasmic domain); Integrin beta1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITBG1D; unnamed protein product; very late activation protein, beta polypeptide
Gene Aliases: CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA; VLAB
UniProt ID: (Human) P05556
Entrez Gene ID: (Human) 3688
magnesium ion binding
fibronectin binding
protease binding
actin binding
obsolete cell adhesion receptor activity
integrin binding
calcium ion binding
protein binding
coreceptor activity
protein kinase binding
C-X3-C chemokine binding
signaling receptor activity
laminin binding
macromolecular complex binding
cadherin binding
protein heterodimerization activity
cell adhesion molecule binding
collagen binding involved in cell-matrix adhesion
integrin binding involved in cell-matrix adhesion
protein tyrosine kinase binding
establishment of mitotic spindle orientation
angiogenesis
phagocytosis
cellular defense response
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
calcium-independent cell-matrix adhesion
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
myoblast fusion
regulation of collagen catabolic process
positive regulation of fibroblast migration
response to muscle activity
cell migration
CD40 signaling pathway
cell projection organization
lamellipodium assembly
B cell differentiation
extracellular matrix organization
positive regulation of cell migration
cell-substrate adhesion
receptor internalization
cell adhesion mediated by integrin
cell-cell adhesion mediated by integrin
heterotypic cell-cell adhesion
wound healing, spreading of epidermal cells
maintenance of blood-brain barrier
positive regulation of apoptotic process
stress fiber assembly
positive regulation of GTPase activity
myoblast differentiation
positive regulation of fibroblast growth factor receptor signaling pathway
positive regulation of angiogenesis
negative regulation of vasoconstriction
mesodermal cell differentiation
regulation of developmental process
modulation of synaptic transmission
leukocyte tethering or rolling
positive regulation of protein kinase B signaling
positive regulation of glutamate uptake involved in transmission of nerve impulse
cellular response to low-density lipoprotein particle stimulus
basement membrane organization
positive regulation of wound healing
cell-cell adhesion
regulation of spontaneous synaptic transmission
reactive gliosis
positive regulation of vascular endothelial growth factor signaling pathway
positive regulation of protein localization to plasma membrane
regulation of synapse pruning
negative regulation of anoikis
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