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Invitrogen
NLGN3 Monoclonal Antibody (N110/29), PE
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FIGURE: 1 / 3
NLGN3 Antibody (MA5-45700) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Neuroligin 3 monoclonal antibody (Product # MA5-45700) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min RT, 5 min RT. Localization: Cell Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) P... View More
Product Details
MA5-45700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N110/29
Immunogen
Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3.
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Mouse: 99% identity (118/119 amino acids identical). Human: 98% identity (116/119 amino acids identical) ~60% identity with Neuroligin-1. ~40% identity with Neuroligin-2.
1 µg/mL of MA5-45700 was sufficient for detection of Neuroligin 3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 110kDa. Does not cross-react with Neuroligin-1, -2, -4 or -4.
This antibody was formerly sold as clone S110-29.
Target Information
This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ASPGX1; AUTSX1; Gliotactin homolog; neuroligin 3 protein; Neuroligin-3; unnamed protein product
Gene Aliases: A230085M13Rik; HNL3; KIAA1480; NL3; NLGN3
UniProt ID: (Human) Q9NZ94, (Mouse) Q8BYM5, (Rat) Q62889
Entrez Gene ID: (Human) 54413, (Mouse) 245537, (Rat) 171297
regulation of respiratory gaseous exchange by neurological system process
receptor-mediated endocytosis
cell adhesion
neuron cell-cell adhesion
chemical synaptic transmission
synapse assembly
learning
adult behavior
social behavior
synaptic vesicle endocytosis
axon extension
modulation of synaptic transmission
synapse organization
positive regulation of synapse assembly
positive regulation of synaptic transmission, glutamatergic
rhythmic synaptic transmission
inhibitory postsynaptic potential
vocalization behavior
postsynaptic membrane assembly
presynaptic membrane assembly
presynapse assembly
positive regulation of glutamate receptor signaling pathway
positive regulation of excitatory postsynaptic potential
visual learning
oligodendrocyte differentiation
regulation of synaptic transmission, glutamatergic
excitatory postsynaptic potential
positive regulation of dendritic spine development
regulation of dendritic spine morphogenesis
positive regulation of inhibitory postsynaptic potential
regulation of long-term synaptic potentiation
positive regulation of protein localization to synapse
regulation of N-methyl-D-aspartate selective glutamate receptor activity
regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
regulation of terminal button organization
positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
metabolic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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