Guide to Digital PCR - From Education to Workflow

We’ve been sharing with you all things digital PCR. From our six-part educational series, which focuses on various aspects of digital PCR to our most recent hands-on complete digital PCR workflow. With these resources handy, we hope to equip you with the tools needed to understand digital PCR, how you can use it for various applications, and a step by step video to guide you through your experiment.

Go Digital PCR: Pt 1 – Next Generation Quantification
We begin our six-part series introducing you to digital PCR (dPCR), what makes it next generation technology, and how it compares to Real-Time Quantitative PCR (qPCR) and traditional PCR.

Go Digital PCR: Pt 2 – QuantStudio™ 3D
In this edition, we’ll take a look at how the QuantStudio™ 3D Digital PCR system offers simplicity, affordability, and most importantly, absolute quantification attainability.

Go Digital PCR: Pt 3 – Copy Number Variation
Part 3 explores how dPCR is enabling researchers’ abilities to achieve precise copy number variation (CNV) analysis, helping detect and quantify small percent copy number differences with a high degree of precision.

Go Digital PCR: Pt 4 – NGS Libraries
In the fourth segment we share with you how dPCR enables highly precise next-generation sequencing (NGS) library quantification.

Go Digital PCR: Pt 5 – Rare Mutations
In our fifth segment we share with you how dPCR is capable of detecting and quantifying rare mutations for low-prevalence targets in cancer research samples.

Go Digital PCR: Pt 6 – Emerging Analyses
This is the final segment in our six-part series introducing you to dPCR and how it aids absolute quantitation and rare sequence detection in lesser used applications.

Hands-on With Digital PCR – QuantStudio 3D Digital PCR Workflow Video
The workflow for the QuantStudio 3D digital PCR system was recently updated and posted on Thermo Fisher Scientific’s YouTube channel. Take a look at the workflow in the video below, and let us know if you have any questions in the comments section.

We hope you’ve enjoyed our educational series and our workflow video. If you have any questions or would like to learn more about a specific topic, please be sure to let us know in the comments section below.