SuperScript™ III Platinum™ One-Step qRT-PCR Kit w/ROX

Catalog number:  11745100

 Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Our One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient, high-throughput one-step format. One-Step qRT-PCR kits with ROX include:

–SuperScript® III Reverse Transcriptase: produces higher yields of full-length cDNA for broader gene representation

–Platinum® Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1)
–ROX reference dye premixed at optimal concentration for ABI instruments

Two kit configurations allow your choice of detection methods:

–SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR Kit provides a highly efficient, cost-effective qRT-PCR system with SYBR Green I detection
–SuperScript® III Platinum® One-Step qRT-PCR Kit provides sensitive, specific detection for use with LUX™ fluorogenic primers or dual-labeled probes (e.g. TaqMan®) (Figure 2)

Contents and Storage:
SuperScript® III Platinum® SYBR® Green One-Step qRT-PCR Kit with ROX includes SuperScript® III RT/Platinum® Taq Mix, 2X Reaction Mix (containing SYBR® Green I, ROX Reference Dye, dNTPs, and MgSO4), and 50 mM MgSO4. SuperScript® III Platinum® One-Step qRT-PCR Kit with ROX includes SuperScript® III RT/Platinum® Taq Mix, 2X Reaction Mix (containing ROX Reference Dye, dNTPs and MgSO4), and 50 mM MgSO4. Store all components at -20°C. Store ROX Reference dye in the dark. Guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 1-Step qRT-PCR
Detection Method: Primer-Probe Detection
Reaction Speed: Standard
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System
Sample Type (General): RNA
Product Line: Platinum®, SuperScript™
Product Size: 100 reactions
Shipping Condition: Dry Ice

Contents & storage

• 100 µl SuperScript® III/Platinum® Taq Mix
• 2 × 1.25 ml 2X Reaction Mix with ROX
• 1 ml Magnesium Sulfate (50 mM)

Store components in the dark at -20°C.


Manuals & protocols