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Invitrogen

TLR4/MD-2 Complex Monoclonal Antibody (MTS510), PE, eBioscience™

11 Published Figures
30 References
View all (9) TLR4/MD-2 antibodies

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Product Details

12-9924-81

Applications
Tested Dilution
Publications

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test
View 28 publications 28 publications
Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MTS510

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

50 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466263

Product Specific Information

Description: The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. At least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. In the mouse, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages. Incubation of peritoneal macrophages with LPS results in down regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, both of which have been well characterized as hyporesponders to LPS.

The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex. Please contact eBioscience Technical Support for further information.

Applications Reported: MTS510 has been reported for use in flow cytometric analysis.

Applications Tested: The MTS510 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: ARMD10; cytokine; hToll; lipopolysaccharide response; ly-96; MD-2; MD-2B; myeloid differentiation factor-2; myeloid differentiation protein-2; protein MD-2; TLR-4; TLR4; Tlr4; similar to Toll/IL-1 superfamily; TOLL; toll homolog; Toll like receptor 4; unnamed protein product

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Gene Aliases: ESOP-1; Lps; Ly87; MD-2; MD2; Ran/M1; Rasl2-8

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UniProt ID: (Mouse) Q9JHF9, (Mouse) Q9QUK6

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Entrez Gene ID: (Mouse) 17087, (Mouse) 21898

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Function(s)
lipopolysaccharide binding lipopolysaccharide receptor activity protein binding Toll-like receptor 4 binding transmembrane signaling receptor activity receptor binding signaling receptor activity identical protein binding phosphatidylinositol 3-kinase binding protein heterodimerization activity
Process(es)
positive regulation of cytokine production regulation of cytokine-mediated signaling pathway pattern recognition receptor signaling pathway toll-like receptor signaling pathway lipopolysaccharide-mediated signaling pathway positive regulation of lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide detection of lipopolysaccharide positive regulation of tumor necrosis factor production toll-like receptor 4 signaling pathway innate immune response cellular response to lipopolysaccharide microglial cell activation activation of innate immune response wound healing involved in inflammatory response immune effector process B cell proliferation involved in immune response nitric oxide production involved in inflammatory response regulation of dendritic cell cytokine production MyD88-dependent toll-like receptor signaling pathway innate immune response-activating signal transduction growth plate cartilage morphogenesis leukotriene metabolic process phagocytosis inflammatory response immune response response to oxidative stress signal transduction I-kappaB kinase/NF-kappaB signaling activation of NF-kappaB-inducing kinase activity I-kappaB phosphorylation response to bacterium gene expression positive regulation of platelet activation positive regulation of gene expression astrocyte development microglia differentiation positive regulation of smooth muscle cell migration neurogenesis signal transduction involved in regulation of gene expression positive regulation of cell migration positive regulation of B cell proliferation regulation of tumor necrosis factor production negative regulation of interferon-gamma production negative regulation of interleukin-17 production negative regulation of interleukin-23 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-13 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of stress-activated MAPK cascade regulation of toll-like receptor 4 signaling pathway TRIF-dependent toll-like receptor signaling pathway cellular response to platelet-derived growth factor stimulus macrophage activation defense response to bacterium positive regulation of macrophage activation positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling leukocyte activation positive regulation of MHC class II biosynthetic process positive regulation of nitric oxide biosynthetic process response to ethanol mast cell activation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade positive regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation regulation of inflammatory response positive regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium detection of temperature stimulus involved in sensory perception of pain detection of mechanical stimulus involved in sensory perception of pain NK T cell activation detection of stimulus bone morphogenesis intestinal epithelial structure maintenance positive regulation of macrophage cytokine production ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway response to fatty acid cellular response to lipoteichoic acid cellular response to mechanical stimulus cellular response to interferon-gamma negative regulation of cold-induced thermogenesis cellular response to oxidised low-density lipoprotein particle stimulus negative regulation of cytokine production involved in inflammatory response positive regulation of cytokine production involved in inflammatory response positive regulation of NLRP3 inflammasome complex assembly positive regulation of reactive oxygen species biosynthetic process positive regulation of cellular response to macrophage colony-stimulating factor stimulus positive regulation of matrix metallopeptidase secretion cellular response to beta-amyloid positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of extrinsic apoptotic signaling pathway
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