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Invitrogen
CD202b (TIE2) Monoclonal Antibody (TEK4), Functional Grade, eBioscience™
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-Functional-Grade-Flow-20170216130641.jpg?time=20220826 "CD202b (TIE2) Antibody in Flow Cytometry (Flow)")
-Functional-Grade-Flow-20170216130641.jpg?time=20220826)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
-Functional-Grade-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD202b (TIE2) Antibody (16-5987-82) in Flow")
.jpg?time=20220826?time=20220826 "CD202b (TIE2) Antibody (16-5987-82) in IHC")
Product Details
16-5987-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TEK4
Conjugate
Functional Grade
Functional Grade
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The TEK4 monoclonal antibody reacts with mouse Tie-2, also known as CD202. A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. Long-term multilineage repopulating cells were detected in Tie-2^+, Lineage^-, c-Kit^+, Sca1^+ cells but not in Tie-2^-, Lineage^-, c-Kit^+, Sca1^+ cells.
Applications Reported: The TEK4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The TEK4 antibody has been tested by blocking of staining with fluorochrome conjugated TEK4 on mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD202b antigen; cell surface receptor homolog; EC 2.7.10; EC 2.7.10.1; endothelial tyrosine kinase; endothelial-specific receptor tyrosine kinase; p140 TEK; TEKT1; Tektin-1; tunica interna endothelial cell kinase; tyrosine kinase with Ig and EGF homology domains-2; tyrosine-protein kinase receptor TEK; tyrosine-protein kinase receptor TIE-2
Gene Aliases: Cd202b; Hyk; STK1; Tie-2; Tie2
UniProt ID: (Mouse) Q02858
Entrez Gene ID: (Mouse) 21687
angiogenesis
patterning of blood vessels
vasculogenesis
response to hypoxia
positive regulation of protein phosphorylation
endothelial cell proliferation
regulation of endothelial cell proliferation
sprouting angiogenesis
positive regulation of cytokine production involved in immune response
cell-matrix adhesion
cell surface receptor signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
heart development
positive regulation of signal transduction
positive regulation of endothelial cell migration
negative regulation of angiogenesis
peptidyl-tyrosine phosphorylation
hemopoiesis
regulation of cell migration
positive regulation of vascular endothelial growth factor receptor signaling pathway
cell-substrate adhesion
response to retinoic acid
regulation of establishment or maintenance of cell polarity
positive regulation of peptidyl-serine phosphorylation
substrate adhesion-dependent cell spreading
positive regulation of protein import into nucleus
negative regulation of apoptotic process
positive regulation of MAPK cascade
response to estrogen
regulation of angiogenesis
positive regulation of angiogenesis
positive regulation of cell adhesion
Tie signaling pathway
branching morphogenesis of an epithelial tube
positive regulation of multicellular organismal process
positive regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
heart trabecula formation
positive regulation of ERK1 and ERK2 cascade
cellular response to mechanical stimulus
glomerulus vasculature development
cell-cell adhesion
regulation of NIK/NF-kappaB signaling
regulation of intracellular signal transduction
positive regulation of intracellular signal transduction
regulation of endothelial cell apoptotic process
negative regulation of endothelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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