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Invitrogen

CD202b (TIE2) Monoclonal Antibody (TEK4), PE, eBioscience™

21 Published Figures
43 References
View all (53) TIE2 (TEK) antibodies

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Product Details

12-5987-82

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Flow Cytometry (Flow)

2 µg/test
View 41 publications 41 publications
Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TEK4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466100

Product Specific Information

Description: The TEK4 monoclonal antibody reacts with mouse Tie-2, also known as CD202. A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. Long-term multilineage repopulating cells were detected in Tie-2^+, Lineage^-, c-Kit^+, Sca1^+ cells but not in Tie-2^-, Lineage^-, c-Kit^+, Sca1^+ cells.

Applications Reported: The TEK4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The TEK4 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: Angiopoietin-1 receptor; CD202b; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; HYK; mTIE2; p140 TEK; STK1; TEKT1; Tektin-1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2

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Gene Aliases: AA517024; Cd202b; Hyk; STK1; Tek; Tie-2; Tie2

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UniProt ID: (Mouse) Q02858

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Entrez Gene ID: (Mouse) 21687

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Function(s)
nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding transmembrane signal receptor
Process(es)
angiogenesis patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation endothelial cell proliferation regulation of endothelial cell proliferation sprouting angiogenesis positive regulation of cytokine secretion involved in immune response protein phosphorylation cell-matrix adhesion transmembrane receptor protein tyrosine kinase signaling pathway heart development positive regulation of endothelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling phosphorylation single organismal cell-cell adhesion negative regulation of angiogenesis peptidyl-tyrosine phosphorylation hemopoiesis regulation of cell migration positive regulation of vascular endothelial growth factor receptor signaling pathway response to retinoic acid regulation of establishment or maintenance of cell polarity positive regulation of peptidyl-serine phosphorylation substrate adhesion-dependent cell spreading intracellular signal transduction positive regulation of protein import into nucleus negative regulation of apoptotic process positive regulation of phosphatidylinositol 3-kinase activity response to estrogen regulation of angiogenesis positive regulation of angiogenesis positive regulation of cell adhesion protein autophosphorylation Tie signaling pathway protein oligomerization positive regulation of focal adhesion assembly positive regulation of protein kinase B signaling heart trabecula formation positive regulation of ERK1 and ERK2 cascade regulation of NIK/NF-kappaB signaling positive regulation of intracellular signal transduction positive regulation of actin cytoskeleton reorganization negative regulation of endothelial cell apoptotic process
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