Thermo Scientific™

NeutrAvidin Protein

Catalog number: 31000
Thermo Scientific™

NeutrAvidin Protein

Catalog number: 31000
Catalog Number
31000
Unit Size
10 mg
Price (USD)
Price: 219.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
100 mg
Price (USD)
Price: 960.00
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Quantity
Catalog Number
Unit Size
1000 mg
Price (USD)
Price: 3,170.00
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
3100010 mg
Price: 219.00
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***
31050100 mg
Price: 960.00
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***
310551000 mg
Price: 3,170.00
Your Price:
***
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Thermo Scientific™ NeutrAvidin Protein is a specially-prepared form of avidin that decreases background in biotin-binding.

Features include:
Near-neutral isoelectric point—pI = 6.3, more neutral than native avidin
Nearly devoid of glycosylation—decreased possibility of lectin binding compared to native avidin
No RYD recognition sequence—no known off-target binding domains like streptavidin
Affordable—significantly less expensive than streptavidin

NeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified NeutrAvidin Protein provides exceptional performance in western blot, ELISA, and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin.

Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles, and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin-binding proteins. The specific activity for biotin binding is approximately 14 µg/mg of protein, which is near the theoretical maximum activity.

Related products
NeutrAvidin™ Protein, Horseradish Peroxidase Conjugated
NeutrAvidin™ Protein, Alkaline Phosphatase Conjugated
NeutrAvidin™ Protein, Fluorescein Conjugated
NeutrAvidin™ Protein, Maleimide-Activated

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
10 mg
Label or Dye
Unlabeled
Target Molecule
Biotin
Form
Solid
Protein Subtype
Avidin

Contents & Storage

Upon receipt store product 4°C. Product is shipped at ambient temperature.

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