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CD54 (ICAM-1) Monoclonal Antibody (1A29), PerCP-eFluor™ 710, eBioscience™
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FIGURE: 1 / 1
CD54 (ICAM-1) Antibody (46-0540-80) in Flow
Staining of rat splenocytes with 0.06 µg of Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or 0.06 µg of Anti-Rat CD54 (ICAM-1) PerCP-eFluor® 710 (purple histogram). Cells in the lymphocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
46-0540-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
1A29
Conjugate
PerCP-eFluor™ 710
PerCP-eFluor™ 710
PerCP-eFluor™ 710
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 1A29 monoclonal antibody reacts with rat CD54, which is also known as intercellular adhesion molecule 1 (ICAM-1). In the immune system, this type I immunoglobulin (Ig)-like transmembrane protein is expressed on lymphocytes, macrophages, dendritic cells, monocytes, granulocytes, and thymic stromal cells. CD54 is also present on endothelial cells and such non-immune cells as hepatocytes, cardiomyocytes, and vascular smooth muscle cells. In addition, reports demonstrate that CD54 expression can be induced in vitro by proinflammatory cytokines, including IFN-gamma, IL-1, CCL2, or TNF-alpha. CD54 plays important roles in transendothelial migration of leukocytes to sites of inflammation and in T cell interactions with antigen presenting cells. Studies indicate that CD54 mediates these functions by binding to lymphocyte function-associated molecule (LFA-1; also known as CD11a/CD18).
The 1A29 antibody has been reported to block interactions between CD54 and LFA-1.
Applications Reported: This 1A29 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 1A29 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Human rhinovirus receptor; ICAM-1; Ly 47; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells
Gene Aliases: CD54; ICAM; RICAM-I
UniProt ID: (Rat) Q00238
Entrez Gene ID: (Rat) 25464
ovarian follicle development
response to hypoxia
response to amphetamine
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
acute inflammatory response to antigenic stimulus
T cell antigen processing and presentation
positive regulation of cellular extravasation
immune response
cell adhesion
leukocyte cell-cell adhesion
I-kappaB kinase/NF-kappaB signaling
regulation of cell shape
response to xenobiotic stimulus
response to ionizing radiation
response to sulfur dioxide
membrane to membrane docking
positive regulation of actin filament polymerization
cellular response to nutrient levels
response to lipopolysaccharide
response to insulin
cell adhesion mediated by integrin
cell-cell adhesion mediated by integrin
response to gonadotropin
response to amino acid
positive regulation of GTPase activity
positive regulation of nitric oxide biosynthetic process
response to ethanol
positive regulation of vasoconstriction
regulation of activated T cell proliferation
response to copper ion
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
negative regulation of calcium ion transport
establishment of endothelial barrier
leukocyte adhesion to vascular endothelial cell
positive regulation of T cell apoptotic process
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to alkaloid
cellular response to glucose stimulus
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to hypoxia
cellular response to dexamethasone stimulus
T cell extravasation
cellular senescence
establishment of endothelial intestinal barrier
establishment of Sertoli cell barrier
cell-cell adhesion
regulation of ruffle assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
response to 2-O-acetyl-1-O-hexadecyl-sn-glycero-3-phosphocholine
cellular response to beta-amyloid
positive regulation of leukocyte adhesion to vascular endothelial cell
cellular response to leukemia inhibitory factor
negative regulation of endothelial cell apoptotic process
Disclaimer
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