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CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eFluor™ 660, eBioscience™
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FIGURE: 1 / 45
CD324 (E-Cadherin) Antibody (50-3249-80) in ICC/IF
Immunofluorescence analysis of CD324 (E-Cadherin) was performed using 70 % confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), eFluor™ 660, eBioscience™ (Product # 50-3249-82) at 2 ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
50-3249-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse
Published species
Hamster,
Human,
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
DECMA-1
Conjugate
eFluor™ 660
eFluor™ 660
eFluor™ 660
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton.
Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers.
E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided.
The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells.
Applications Reported: This DECMA-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This DECMA-1 antibody has been tested by flow cytometric analysis of MDCK cell line. Optimal staining is achieved by intracellular staining as protein turnover can result in variable surface staining. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); cadherin e; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CDH1; cell-CAM 120/80; E-cadherin; E-cadherin 1; Ecadherin; epididymis secretory sperm binding protein; Epithelial cadherin; unnamed protein product; uvomorulin
Gene Aliases: ARC-1; BCDS1; Cadherin-1; CD324; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO; Uvomorulin
UniProt ID: (Human) P12830, (Dog) F1PAA9, (Mouse) P09803
Entrez Gene ID: (Human) 999, (Dog) 442858, (Mouse) 12550
calcium ion binding
protein binding
beta-catenin binding
ankyrin binding
GTPase activating protein binding
identical protein binding
gamma-catenin binding
cadherin binding
cell adhesion molecule binding
cell adhesion mediator activity
protein tyrosine kinase binding
cytoskeletal protein binding
protein phosphatase binding
protein domain specific binding
alpha-catenin binding
binding, bridging
protein binding involved in cell-cell adhesion
cadherin
cell morphogenesis
desmosome assembly
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
response to xenobiotic stimulus
response to toxic substance
regulation of gene expression
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
pituitary gland development
negative regulation of cell-cell adhesion
negative regulation of cell migration
negative regulation of axon extension
neuron projection development
cell junction assembly
adherens junction organization
positive regulation of protein import into nucleus
cell-cell adhesion mediated by cadherin
positive regulation of transcription, DNA-templated
cellular response to lithium ion
response to heparin
cellular response to indole-3-methanol
protein localization to plasma membrane
cell-cell adhesion
regulation of protein catabolic process at postsynapse, modulating synaptic transmission
response to Gram-positive bacterium
positive regulation of cellular protein localization
in utero embryonic development
trophectodermal cell differentiation
epithelial cell morphogenesis
embryo implantation
sensory perception of sound
negative regulation of protein processing
positive regulation of cell-cell adhesion
actin cytoskeleton organization
regulation of protein localization
uterine epithelium development
decidualization
negative regulation of epithelial cell proliferation
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
establishment of skin barrier
bicellular tight junction assembly
cellular response to amino acid stimulus
negative regulation of canonical Wnt signaling pathway
cochlea development
negative regulation of protein localization to plasma membrane
regulation of protein localization to cell surface
regulation of neuron migration
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