Pierce™ Asp-N Protease, MS Grade

Catalog number: 90053

Thermo Scientific™  Related applications: Protein Mass Spectrometry Analysis

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Description

Thermo Scientific Asp-N Protease, MS Grade, is a highly specific endoproteinase used to improve sequence coverage in mass spectrometry protein identification applications.

Features of MS Grade Asp-N Protease:

• Complementary to tryptic digests—hydrolyzes proteins specifically at the amino side of aspartate and cysteic acid residues
• Increased sequence coverage—better protein characterization results from overlapping peptides with complementary chromatographic, ionization and fragmentation properties
• High specific activity—greater than 20,000 units/mg protein
• N-terminal arginine cleavage specificity—at least 90% for a complex protein sample
• Stable—provided in a lyophilized format

Product Details
This Asp-N is a mass spectrometry (MS)-grade zinc metalloproteinase derived from a mutant strain of Pseudomonas fragi and requires a trace amount of zinc for activity. Asp-N can be used alone or in parallel with trypsin or other proteases to produce protein digests for peptide mapping and protein sequencing. Asp-N protease is suitable for either in-solution or in-gel digestion workflows. This Asp-N enzyme is packaged lyophilized (2 µg).

The endoproteinase AspN cleaves primarily at amino side of aspartate residues and cysteic acid residues that result from the oxidization of cysteine residues, generating a limited number of peptide fragments. Cleavage can also occur at glutamic residues; however, the rate of cleavage at the glutamyl residues is significantly lower than the rate of cleavage at the aspartic acid residues. AspN can efficiently digest protein in 2-20 hours at 37°C. AspN remains active under denaturing conditions such as 1M urea, 2M guanidine·HCl, 0.1% SDS, 2% CHAPS and 10% acetonitrile with optimal activity in the pH range of 6-8. This lyophilized enzyme has a mass of 27 kDa and is stable for 1 year when stored at -20°C.

Applications
• Improved sequence coverage of protein digests
• In-solution digestion of proteins
• In-gel digestion of proteins
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Mass Spectrometry
Final Product: Peptide
For Use With (Equipment): Mass Spectrometer
Product Size: 2 µg
Starting Material: Cell Lysate, Protein samples
Workflow Step: Protein Digestion

Contents & storage

Store at -20°C in a nonfrost-free freezer.

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Manuals & protocols

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