Ion Torrent™

Oncomine™ Tumor Mutation Load Assay, Chef-ready library preparation

Catalog number:  A37910

Related applications:

Ion Torrent™ Next-Generation Sequencing

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The Oncomine Tumor Mutation Load Assay is a targeted next-generation sequencing (NGS) assay that provides an assessment of tumor mutation load and mutation signatures in a simple workflow. The assay provides reagents for library construction from two pools of multiplex PCR primers for preparation of amplicon libraries from formalin-fixed paraffin-embedded (FFPE) tumor samples without the need for a matched normal sample, using 10 ng of DNA per primer pool. This assay is designed to facilitate successful selection and identification of samples most likely to derive responses in cancer immunotherapy research.

• Enables accurate quantitation of somatic mutations to assess tumor mutation load
• Single tumor sample workflow reduces the need for a matched normal sample
• Efficient amplification from challenging samples with AmpliSeq technology
• Streamlined analysis with feature-rich visualization
• Efficient multiplexing of up to eight samples per Ion 540 Chip on the Ion S5 Systems

The Oncomine Tumor Mutation Load Assay, Chef-Ready Library Preparation, includes panel primers and reagents for automated library construction and normalization sufficient for 32 samples. This version of the assay is for automated library preparation using the Ion Chef System. For manual library preparation, please see Cat. No. A37909.

From sample to answer
The Oncomine Tumor Mutation Load Assay comes with optimized informatics and visualization software that provides a sample-to-answer solution. Run metrics can be assessed using a Torrent Suite plugin. The Ion Reporter analysis workflow utilizes a custom variant calling and germline variant filtering algorithm to accurately estimate somatic variants in cancer research samples. A detailed report is provided that includes the normalized mutation load (mutations/MB) and mutation signatures of the somatic variants, including percentage of mutations consistent with UV damage, tobacco smoke damage, de-amination, and specific substitutions.

Learn more about Oncomine Oncology ›


Libraries: Targeted Sequencing Library
Product Line: Oncomine™
Sequencing Type: Genome & DNA Sequencing
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Workflow Step: Library Generation
For Use With (Equipment): Ion GeneStudio S5 System, Ion S5™ XL System
Starting Material: DNA
Throughput: 32 Samples
Type: Tumor Mutation Load Assay

Contents & storage

• 1 Oncomine Tumor Mutation Load Panel, 32 reactions, store at -5 to -30°C.
• 4 cartridges Ion AmpliSeq Chef Reagents DL8, store at -5 to -30°C.
• 4 cartridges Ion AmpliSeq Chef Solutions DL8, store at 2–8°C.
• 4 boxes Ion AmpliSeq Supplies, store at room temperature.
• 1 IonCode in PCR Plates, store at room temperature.