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CD115 (c-fms) Monoclonal Antibody (AFS98), NovaFluor™ Yellow 610, eBioscience™

View all (93) CSF1R (CD115) antibodies

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Product Details

M041T02Y03-A

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.6 µg/test
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

AFS98

Conjugate

NovaFluor™ Yellow 610 NovaFluor™ Yellow 610 NovaFluor™ Yellow 610

Excitation/Emission Max

551/614 nm View spectra spectra

Form

Liquid

Concentration

0.1 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

RRID

AB_3099144

Product Specific Information

Description
The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Tested
The AFS98 antibody has been tested by flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Master mixes
• Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time.
• We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer.

Whole Blood compatibility
• When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.
• See the Bulk Lysis of Human Whole Blood protocol here.
• Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining.

Viability dye compatibility
• NovaFluor dyes are not compatible with DNA intercalating viability dyes.
• Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

CellBlox Plus Blocking Buffer
• This NovaFluor conjugate comes with CellBlox Plus Blocking Buffer (for 25 µg or 25 test products, Cat. No. C001T02F01; for 100 µg or 100 test, Cat. No. C001T03F01), essential for optimal staining.
• Use CellBlox Plus Blocking Buffer in all experiments with NovaFluor conjugates.
• Add 5 µL per sample to antibody cocktails/master mixes (regardless of how many Novafluor-conjugated antibodies are present) before combining with cells.
• CellBlox Plus Blocking Buffer is compatible with either Super Bright Complete Blocking Buffer or Brilliant Stain Buffer and can be used in antibody cocktails/master mixes with those reagents.
• For single-color controls, use 5 µL of CellBlox Plus Blocking Buffer per 100 µL of cell sample (10^3 to 10^8 cells).

NovaFluor conjugates are based on Phiton technology utilizing novel fluorophore-containing nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Our internal testing shows that NovaFluor Yellow 610 non-specifically stains B cells in SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference.

Excitation: 551 nm; Emission: 614 nm; Laser: 561 nm (Yellow) Laser

Target Information

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Bioinformatics

Protein Aliases: c-fms; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; OTTHUMP00000224093; proto-oncogene c-Fms; proto-oncogene fms; unnamed protein product

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Gene Aliases: CD115; CSF-1R; Csfmr; Fim-2; Fim2; Fms; M-CSF-R; M-CSFR

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UniProt ID: (Mouse) P09581

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Entrez Gene ID: (Mouse) 12978

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Function(s)
protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity macrophage colony-stimulating factor receptor activity protein binding ATP binding growth factor binding protein phosphatase binding cytokine binding protein homodimerization activity
Process(es)
microglial cell activation neutrophil homeostasis positive regulation of protein phosphorylation myeloid leukocyte differentiation response to ischemia response to stress signal transduction transmembrane receptor protein tyrosine kinase signaling pathway axon guidance cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell shape anatomical structure morphogenesis positive regulation of gene expression positive regulation of macrophage chemotaxis negative regulation of myotube differentiation microglia development cell migration peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway telencephalon development dentate gyrus development olfactory bulb development forebrain neuron differentiation regulation of cell morphogenesis hemopoiesis osteoclast differentiation positive regulation of cell migration positive regulation of bone mineralization negative regulation of epithelial cell differentiation ruffle organization positive regulation of chemokine production positive regulation of tumor necrosis factor production regulation of mononuclear cell proliferation regulation of actin cytoskeleton organization monocyte homeostasis cellular response to macrophage colony-stimulating factor stimulus macrophage colony-stimulating factor signaling pathway positive regulation of tyrosine phosphorylation of STAT protein negative regulation of apoptotic process regulation of MAPK cascade positive regulation by host of viral process regulation of bone resorption cell-cell junction maintenance positive regulation of osteoclast differentiation protein autophosphorylation positive regulation of lymphocyte proliferation positive regulation of protein kinase B signaling positive regulation of protein tyrosine kinase activity microglial cell proliferation positive regulation of astrocyte activation positive regulation of ERK1 and ERK2 cascade positive regulation of leukocyte proliferation cellular response to cytokine stimulus positive regulation of macrophage proliferation positive regulation of cell cycle G1/S phase transition negative regulation of platelet formation regulation of macrophage migration positive regulation of macrophage migration neuron projection extension positive regulation of cell motility positive regulation of myoblast proliferation
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