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Invitrogen
E-cadherin Monoclonal Antibody (7H12)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-15711
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
7H12
Immunogen
Purified recombinant fragment of human CDH1 expressed in E. Coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15711 targets CDH1 in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.
The MA5-15711 immunogen is purified recombinant fragment of human CDH1 expressed in E. Coli.
MA5-15711 detects CDH1 which has a predicted molecular weight of approximately 135kDa.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); cadherin e; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; cell-CAM 120/80; E-cadherin; E-cadherin 1; Ecadherin; epididymis secretory sperm binding protein; epithelial cadherin; unnamed protein product; uvomorulin
Gene Aliases: ARC-1; BCDS1; CD324; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO
UniProt ID: (Human) P12830, (Mouse) P09803
Entrez Gene ID: (Human) 999, (Mouse) 12550
calcium ion binding
protein binding
beta-catenin binding
ankyrin binding
GTPase activating protein binding
identical protein binding
gamma-catenin binding
cadherin binding
cell adhesion molecule binding
cell adhesion mediator activity
protein tyrosine kinase binding
cytoskeletal protein binding
protein phosphatase binding
protein domain specific binding
alpha-catenin binding
binding, bridging
protein binding involved in cell-cell adhesion
cell morphogenesis
desmosome assembly
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
response to xenobiotic stimulus
response to toxic substance
regulation of gene expression
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
pituitary gland development
negative regulation of cell-cell adhesion
negative regulation of cell migration
negative regulation of axon extension
neuron projection development
cell junction assembly
adherens junction organization
positive regulation of protein import into nucleus
cell-cell adhesion mediated by cadherin
positive regulation of transcription, DNA-templated
cellular response to lithium ion
response to heparin
cellular response to indole-3-methanol
protein localization to plasma membrane
cell-cell adhesion
regulation of protein catabolic process at postsynapse, modulating synaptic transmission
response to Gram-positive bacterium
positive regulation of cellular protein localization
in utero embryonic development
trophectodermal cell differentiation
epithelial cell morphogenesis
embryo implantation
sensory perception of sound
negative regulation of protein processing
positive regulation of cell-cell adhesion
actin cytoskeleton organization
regulation of protein localization
uterine epithelium development
decidualization
negative regulation of epithelial cell proliferation
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
establishment of skin barrier
bicellular tight junction assembly
cellular response to amino acid stimulus
negative regulation of canonical Wnt signaling pathway
cochlea development
negative regulation of protein localization to plasma membrane
regulation of protein localization to cell surface
regulation of neuron migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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