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Invitrogen

ATG5 Polyclonal Antibody, HRP

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ATG5 Polyclonal Antibody, HRP

Product Details

PA5-22857

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

Flow Cytometry (Flow)

Assay-dependent
-

ELISA (ELISA)

Assay-Dependent
-

Immunoprecipitation (IP)

Assay-dependent
-

Radioimmune Assays (RIA)

Assay-dependent
-

Proximity Ligation Assay (PLA) (PLA)

Assay-dependent
-

Immunomicroscopy (IM)

Assay-dependent
-
Product Specifications

Species Reactivity

Bovine, Fruit fly, Fish, Guinea pig, Human, Mouse, Non-human primate, Pig, Reptile, Rat, Xenopus, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide of an N-terminal region of the human ATG5 protein (within residues 1-50).
3D Epitope / Immunogen

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

0.92 mg/mL

Amount

92 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11157872

Product Specific Information

In Western Blot, a band is seen ~56 kDa representing the ATG5-ATG12 complex, the molecular weight of human ATG5 is ~33 kDa.

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Suggested positive control: mouse wildtype ES cell lysate.

Target Information

ATG5 (Autophagy Related 5) is an important element for autophagy and may play an important role in the apoptotic process. ATG5 is also involved in other cellular processes that include mitochondrial quality control after oxidative damage, negative regulation of the innate anti-viral immune response, lymphocyte development and proliferation, MHC II antigen presentation, and adipocyte differentiation. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. ATG5 contributes to autophagic cell death by interacting with Fas-associated protein with death domain (FADD). The ATG5-ATG12 conjugate forms a cup-shaped isolation membrane that then detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may play a role in the apoptotic process, possibly within the modified cytoskeleton. Further, APG5 expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. The APG5-APG12 conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Diseases associated with ATG5 dysfunction include spinocerebellar ataxia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alternatively spliced; APG 5; APG5; APG5 autophagy 5 like (APG5L); APG5 autophagy 5-like; apoptosis-specific protein; Apoptosis-specific protein (ASP); AT5G17290; ATATG5; ATG5; ATG5 autophagy related 5 homolog; Atg5-PA; AUTOPHAGY 5; autophagy 5-like protein; autophagy protein 5; autophagy related 5-like protein; autophagy-specific gene 5; CG1643-PA; LD34980p; MKP11.20; MKP11_20; similar to S. cerevisiae ATG5 autophagy related 5; unnamed protein product

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Gene Aliases: 2010107M05Rik; 3110067M24Rik; APG5; APG5-LIKE; APG5L; ASP; Atg-5; ATG5; Atg5l; CG1643; DmAtg5; Dmel\CG1643; Dmel_CG1643; hAPG5; Paddy; SCAR25; XELAEV_18027227mg; zgc:100934

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UniProt ID: (Pig) Q3MQ04, (Human) Q9H1Y0, (Bovine) Q3MQ24, (Fruit fly) Q9W3R7, (Rat) Q3MQ06, (Mouse) Q99J83

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Entrez Gene ID: (Pig) 100739102, (Human) 9474, (Bovine) 532686, (Fruit fly) 31666, (Guinea pig) 100735170, (Rat) 365601, (Mouse) 11793, (Zebrafish) 494180, (Xenopus) 443756

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Function(s)
protein binding Atg8 ligase activity membrane trafficking regulatory protein
Process(es)
immune system process autophagy apoptotic process regulation of cilium assembly autophagosome assembly mitophagy cellular response to nitrogen starvation response to iron(II) ion positive regulation of autophagy macroautophagy negative regulation of type I interferon production piecemeal microautophagy of nucleus aggrephagy negative regulation of apoptotic process negative regulation of programmed cell death post-translational protein modification negative regulation of innate immune response negative regulation of defense response to virus chaperone-mediated autophagy positive regulation of stress granule assembly cellular response to nitrosative stress axonal transport regulation of postsynaptic membrane neurotransmitter receptor levels postsynaptic modulation of chemical synaptic transmission regulation of autophagosome maturation response to fluoride negative regulation of autophagic cell death positive regulation of viral translation blood vessel remodeling response to fungus antigen processing and presentation of endogenous antigen negative regulation of protein ubiquitination negative stranded viral RNA replication vasodilation response to drug negative thymic T cell selection otolith development negative regulation of phagocytosis regulation of release of sequestered calcium ion into cytosol ventricular cardiac muscle cell development heart contraction protein lipidation involved in autophagosome assembly positive regulation of mucus secretion autophagy of host cells involved in interaction with symbiont negative regulation of reactive oxygen species metabolic process negative regulation of histone H4-K16 acetylation mitophagy C-terminal protein lipidation cellular response to starvation larval midgut cell programmed cell death nucleophagy glycophagy regulation of cytokine production involved in immune response response to xenobiotic stimulus innate immune response defense response to virus regulation of organelle assembly regulation of reactive oxygen species metabolic process nervous system development heart development regulation of skeletal muscle tissue regeneration
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