Penicillin-Streptomycin (10,000 U/mL)

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Citations & References (34)

Gibco™

Penicillin-Streptomycin (10,000 U/mL)

Name: Penicillin-Streptomycin (10,000 U/mL)
SKU: 15140122

この溶液には、10,000ユニット／mLのペニシリンと10,000 µg/mLのストレプトマイシンが含まれます。抗生物質のペニシリンとストレプトマイシンは、グラム陽性菌とグラム陰性菌に対する効果的な組み合わせ作用により、細胞培養の細菌汚染を防ぎます。ペニシリンは菌類青カビから独自に精製され、細菌細胞壁の代謝回転に直接干渉することで作用し、細胞壁をさらに変化させる酵素の放出を誘発することによって間接的に作用します。ストレプトマイシンは詳細を見る

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製品番号（カタログ番号）	数量
15140122 または、製品番号15140-122	100 mL
15140148 または、製品番号15140-148	20 mL
15140163 または、製品番号15140-163	20 x 100 mL

3 オプション

製品番号（カタログ番号） 15140122

または、製品番号15140-122

価格（JPY）

8,400

Each

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数量:

100 mL

この溶液には、10,000ユニット／mLのペニシリンと10,000 µg/mLのストレプトマイシンが含まれます。抗生物質のペニシリンとストレプトマイシンは、グラム陽性菌とグラム陰性菌に対する効果的な組み合わせ作用により、細胞培養の細菌汚染を防ぎます。ペニシリンは菌類青カビから独自に精製され、細菌細胞壁の代謝回転に直接干渉することで作用し、細胞壁をさらに変化させる酵素の放出を誘発することによって間接的に作用します。ストレプトマイシンは、ストレプトマイセスグリセウスから精製されました。細菌リボソームの30Sサブユニットに結合することで作用し、タンパク質合成を阻害し、感受性細菌を死滅させます。

当社は、粉末と液体の両方で幅広い抗生物質および抗真菌剤を提供しています。各製品タイプの詳細については、以下をご覧ください。

•細胞培養用抗生物質
• 選択用抗生物質（推奨される使用濃度を含む）

細胞培養における抗生物質および抗真菌剤の詳しい使用方法と、培養物の汚染除去に関するガイドラインについては、こちらをご覧ください。

研究用途にのみご使用ください。診断目的には使用できません。

仕様

濃度100 X

使用対象（アプリケーション）Prevention of Cell Culture Contamination

数量100 mL

品質保持期間12 Months

出荷条件Dry Ice

形状Liquid

製品タイプAntibiotic

無菌性Sterile-filtered

Sterilization MethodSterile-filtered

Unit SizeEach

組成および保存条件

Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

My Penicillin-Streptomycin solution is not colorless. Is this normal?

Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (34)

引用および参考文献

Abstract

Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.

Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; BÃƒÂ¶hm Michael; Nickenig Georg;

Journal:Circulation

PubMed ID:12021231

BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More

Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.

Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,

Journal:J Biol Chem

PubMed ID:22810232

'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More

Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.

Authors:Friedrich U, Blom AM, DahlbÃ¤ck B, Villoutreix BO,

Journal:J Biol Chem

PubMed ID:11316800

'Human activated protein C (APC) is a key component of a natural anticoagulant system that regulates blood coagulation. In vivo, the catalytic activity of APC is regulated by two serpins, alpha1-antitrypsin and the protein C inhibitor (PCI), the inhibition by the latter being stimulated by heparin. We have identified a ... More

Hypoxia induces the activation of the phosphatidylinositol 3-kinase/Akt cell survival pathway in PC12 cells: protective role in apoptosis.

Journal:J Biol Chem

PubMed ID:11294857

'Hypoxia is a common environmental stress that influences signaling pathways and cell function. Several cell types, including neuroendocrine chromaffin cells, have evolved to sense oxygen levels and initiate specific adaptive responses to hypoxia. Here we report that under hypoxic conditions, rat pheochromocytoma PC12 cells are resistant to apoptosis induced by ... More

Electrophoretic profiling of both RNA and protein from a single 250-pL sample.

Authors: Zabzdyr Jennifer L; Lillard Sheri J;

Journal:Anal Chem

PubMed ID:11985318

'A novel approach is described that uses capillary electrophoresis (CE) to electrophoretically sample and separate both protein and RNA from a single injected plug of cell lysate. A 250-pL sample of lysate from Chinese hamster ovary cells (9.6 x 10(7) cells/mL) was hydrodynamically injected into a capillary containing a Tris-based ... More

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