Penicillin-Streptomycin (10,000 U/mL)
Try new Pen-Strep antibiotic with GlutaMAX supplement, a stable alternative to L-glutamine that enhances cell health. Learn more >
Penicillin-Streptomycin (10,000 U/mL)
Penicillin-Streptomycin (10,000 U/mL)
Penicillin-Streptomycin (10,000 U/mL)
Citations & References (43)
Gibco™

Penicillin-Streptomycin (10,000 U/mL)

この溶液には、10,000ユニット／mLのペニシリンと10,000 µg/mLのストレプトマイシンが含まれます。抗生物質のペニシリンとストレプトマイシンは、グラム陽性菌とグラム陰性菌に対する効果的な組み合わせ作用により、細胞培養の細菌汚染を防ぎます。ペニシリンは菌類青カビから独自に精製され、細菌細胞壁の代謝回転に直接干渉することで作用し、細胞壁をさらに変化させる酵素の放出を誘発することによって間接的に作用します。ストレプトマイシンは詳細を見る
テクニカルサポートオーダーサポート
表示を変更buttonViewtableView
製品番号（カタログ番号）数量
15140148
または、製品番号15140-148
20 mL
15140122
または、製品番号15140-122
100 mL
15140163
または、製品番号15140-163
20 x 100 mL
製品番号（カタログ番号） 15140148
または、製品番号15140-148
価格（JPY）
4,800
Each
お問い合わせください ›
数量:
20 mL
この溶液には、10,000ユニット／mLのペニシリンと10,000 µg/mLのストレプトマイシンが含まれます。抗生物質のペニシリンとストレプトマイシンは、グラム陽性菌とグラム陰性菌に対する効果的な組み合わせ作用により、細胞培養の細菌汚染を防ぎます。ペニシリンは菌類青カビから独自に精製され、細菌細胞壁の代謝回転に直接干渉することで作用し、細胞壁をさらに変化させる酵素の放出を誘発することによって間接的に作用します。ストレプトマイシンは、ストレプトマイセスグリセウスから精製されました。細菌リボソームの30Sサブユニットに結合することで作用し、タンパク質合成を阻害し、感受性細菌を死滅させます。

当社は、粉末と液体の両方で幅広い抗生物質および抗真菌剤を提供しています。各製品タイプの詳細については、以下をご覧ください。

•細胞培養用抗生物質
• 選択用抗生物質（推奨される使用濃度を含む）

細胞培養における抗生物質および抗真菌剤の詳しい使用方法と、培養物の汚染除去に関するガイドラインについては、こちらをご覧ください。

研究用途にのみご使用ください。診断目的には使用できません。
仕様
濃度100 X
使用対象（アプリケーション）Prevention of Cell Culture Contamination
数量20 mL
品質保持期間12 Months
出荷条件Dry Ice
形状Liquid
製品タイプAntibiotic
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
Unit SizeEach
組成および保存条件
Storage conditions: -5 to -20°C
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

My Penicillin-Streptomycin solution is not colorless. Is this normal?

Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (43)

引用および参考文献
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; Böhm Michael; Nickenig Georg;
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More
Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx.
Authors: Vargas Leonardo; Nore Beston F; Berglof Anna; Heinonen Juhana E; Mattsson Pekka T; Smith C I Edvard; Mohamed Abdalla J;
Journal:J Biol Chem
PubMed ID:11751885
'Bruton''s tyrosine kinase (Btk), a member of the Tec family of protein-tyrosine kinases, has been shown to be crucial for B cell development, differentiation, and signaling. Mutations in the Btk gene lead to X-linked agammaglobulinemia in humans and X-linked immunodeficiency in mice. Using a co-transfection approach, we present evidence here ... More
Repression of activator protein-1-mediated transcriptional activation by the Notch-1 intracellular domain.
Authors: Chu Jianlin; Jeffries Shawn; Norton Jason E; Capobianco Anthony J; Bresnick Emery H;
Journal:J Biol Chem
PubMed ID:11739397
'Developmental decisions that control cell fate are commonly regulated by the Notch signaling pathway. Activation of transmembrane Notch receptors results in proteolytic liberation of the intracellular domain of Notch, which translocates into the nucleus, binds a repressor (C promoter binding factor 1/RBP-Jkappa, Su(H), and Lag-1 (CSL)), and induces target genes. ... More
A differential role for the mitogen-activated protein kinases in lipopolysaccharide signaling: the MEK/ERK pathway is not essential for nitric oxide and interleukin 1beta production.
Authors: Watters Jyoti J; Sommer Julie A; Pfeiffer Zachary A; Prabhu Usha; Guerra Alma N; Bertics Paul J;
Journal:J Biol Chem
PubMed ID:11786532
'Endotoxin (lipopolysaccharide, LPS) is a component of the outer membrane of Gram-negative bacteria and promotes the activation of macrophages and microglia. Although these cells are highly LPS-responsive, they serve unique tissue-specific functions and exhibit different LPS sensitivities. Accordingly, it was of interest to evaluate whether these biological differences reside in ... More
Cholesteryl ester is transported from caveolae to internal membranes as part of a caveolin-annexin II lipid-protein complex.
Authors: Uittenbogaard Annette; Everson William V; Matveev Sergey V; Smart Eric J;
Journal:J Biol Chem
PubMed ID:11733519
'We previously demonstrated that in Chinese hamster ovary cells scavenger receptor, class B, type I-dependent selective cholesteryl ester uptake occurs in caveolae. In the present study we hypothesized that cholesteryl ester is transported from caveolae through the cytosol to an internal membrane by a caveolin chaperone complex similar to the ... More
View all Penicillin-Streptomycin (10,000 U/mL), 20 mL citations