EGS (ethylene glycol bis(succinimidyl succinate))

Citations & References (5)

Thermo Scientific™

EGS (ethylene glycol bis(succinimidyl succinate))

Thermo Scientific Pierce EGSは、12-原子スペーサーアームの周囲にアミン反応性NHS-エステル末端を含むクロスリンカーで、pH 8.5でヒドロキシルアミンで処理して開裂できます。EGSクロスリンカーの特長：•反応基：NHSエステル（両端）•詳細を見る

製品番号（カタログ番号）	数量
21565	1 g

製品番号（カタログ番号） 21565

価格（JPY）

52,000

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Thermo Scientific Pierce EGSは、12-原子スペーサーアームの周囲にアミン反応性NHS-エステル末端を含むクロスリンカーで、pH 8.5でヒドロキシルアミンで処理して開裂できます。

EGSクロスリンカーの特長：

•反応基：NHSエステル（両端）
• 反応性：アミノ基（第一級アミン）
• 水溶性（DMFまたはDMSOで最初に溶解）、Sulfo-EGSと比較
• 膜透過性、細胞内架橋が可能
• 形成した架橋は37℃で3～6時間ヒドロキシルアミンを使用して、pH 8.5で可逆化可能
• ラクトースデヒドロゲナーゼはEGSで可逆的に架橋した後もその活動の60%を維持します

製品リファレンス：
架橋剤アプリケーションガイド - この製品に関する最近の参考文献を検索します

EGS（エチレングリコールビス（スクシンイミジルスクシネート））

研究用途にのみご使用ください。診断目的には使用できません。

細胞透過性Yes

概要EGS

形状Powder

標識法化学的標識

分子量456.36

PEG化No

製品ラインPierce

数量1 g

反応性部分NHSエステル

出荷条件Ambient

溶解性DMF、DMSO

スペーサーアーム長16.1 Å

水溶性No

化学反応性Amine-Amine

CleavableBy Hydroxylamine

クロスリンカータイプホモ二官能性

フォーマットStandard

製品タイプ架橋剤

スペーサー切断可能、長

Unit SizeEach

組成および保存条件

4℃で保存。室温で出荷

よくあるご質問（FAQ）

Can you provide the shelf-life for EGS (ethylene glycol bis(succinimidyl succinate))?

EGS (ethylene glycol bis(succinimidyl succinate)) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can NHS-diazirine (Cat. No. 26167) be used for CHIP applications?

NHS-diazirine has not been tested for cross-linking in a ChIP application. EGS (Cat. No. 21565) and DSG (Cat. No. 20593) are not used by themselves in ChIP, but rather in combination with formaldehyde. While formaldehyde is good for crosslinking proteins that are in direct contact with DNA, it cannot trap proteins that may be bound in a complex with other proteins but are not in direct contact. Thus EGS or DSG can cross-link proteins to proteins that are in direct contact with DNA and are crosslinked to it by formaldehyde. See this reference for more information

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

引用および参考文献 (5)

引用および参考文献

Abstract

Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosis.

Authors:Keinan N, Tyomkin D, Shoshan-Barmatz V

Journal:Mol Cell Biol

PubMed ID:20937774

'Accumulating evidence implicates that the voltage-dependent anion channel (VDAC) functions in mitochondrion-mediated apoptosis and as a critical player in the release of apoptogenic proteins, such as cytochrome c, triggering caspase activation and apoptosis. The mechanisms regulating cytochrome c release and the molecular architecture of the cytochrome c-conducting channel remain unknown. ... More

Structure-based analysis of VDAC1 protein: defining oligomer contact sites.

Authors:Geula S, Naveed H, Liang J, Shoshan-Barmatz V

Journal:J Biol Chem

PubMed ID:22117062

'The outer mitochondrial membrane protein, the voltage-dependent anion channel (VDAC), is increasingly implicated in the control of apoptosis. Oligomeric assembly of VDAC1 was shown to be coupled to apoptosis induction, with oligomerization increasing substantially upon apoptosis induction and inhibited by apoptosis blockers. In this study, structure- and computation-based selection of ... More

Integrated genome-wide analysis of transcription factor occupancy, RNA polymerase II binding and steady-state RNA levels identify differentially regulated functional gene classes.

Authors:Mokry M, Hatzis P, Schuijers J, Lansu N, Ruzius FP, Clevers H, Cuppen E

Journal:Nucleic Acids Res

PubMed ID:21914722

'Routine methods for assaying steady-state mRNA levels such as RNA-seq and micro-arrays are commonly used as readouts to study the role of transcription factors (TFs) in gene expression regulation. However, cellular RNA levels do not solely depend on activity of TFs and subsequent transcription by RNA polymerase II (Pol II), ... More

LXRa regulates macrophage arginase 1 through PU.1 and interferon regulatory factor 8.

Authors:Pourcet B, Feig JE, Vengrenyuk Y, Hobbs AJ, Kepka-Lenhart D, Garabedian MJ, Morris SM, Fisher EA, Pineda-Torra I

Journal:Circ Res

PubMed ID:21757649

Activation of liver X receptors (LXRs) inhibits the progression of atherosclerosis and promotes regression of existing lesions. In addition, LXRa levels are high in regressive plaques. Macrophage arginase 1 (Arg1) expression is inversely correlated with atherosclerosis progression and is markedly decreased in foam cells within the lesion. ... More

Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation.

Authors:Gogada R, Prabhu V, Amadori M, Scott R, Hashmi S, Chandra D

Journal:J Biol Chem

PubMed ID:21712378

Resveratrol, a naturally occurring phytoalexin, is known to induce apoptosis in multiple cancer cell types, but the underlying molecular mechanisms remain unclear. Here, we show that resveratrol induced p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated translocation of Bax to mitochondria where it underwent oligomerization to initiate apoptosis. Resveratrol treatment promoted ... More